Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P. R. China.
ACS Appl Mater Interfaces. 2022 Aug 24;14(33):37504-37513. doi: 10.1021/acsami.2c10420. Epub 2022 Aug 15.
Dual-emitting polymer dots (dual-Pdots) in the visible and second near-infrared (NIR-II) region can facilitate the high-resolution imaging of the fine structure and improve the signal-to-noise ratio in in vivo imaging. Herein, combining high brightness of Pdots and multi-scale imaging, we synthesized dual-Pdots using a simple nano-coprecipitation method and performed multi-functional imaging of vessels, brown adipose tissue, and bones. Results showed that in vivo blood vessel imaging had a high resolution of up to 5.9 μm and bone imaging had a signal-to-noise ratio of 3.9. Moreover, dual-Pdots can accumulate in the interscapular brown adipose tissue within 2 min with a signal-to-noise ratio of 5.8. In addition, the prepared dual-Pdots can image the lymphatic valves and the frequency of contraction. Our study provides a feasible method of using Pdots as nanoprobes for multi-scale imaging in the fields of metabolic disorders, skeletal system diseases, and circulatory systems.
双发射聚合物点（dual-Pdots）在可见和第二近红外（NIR-II）区域，可以促进精细结构的高分辨率成像，并提高体内成像的信噪比。在此，我们结合了 Pdot 的高亮度和多尺度成像，使用简单的纳米共沉淀法合成了双 Pdot，并进行了血管、棕色脂肪组织和骨骼的多功能成像。结果表明，体内血管成像的分辨率高达 5.9μm，骨成像的信噪比为 3.9。此外，双 Pdot 可以在 2 分钟内积聚在肩胛间棕色脂肪组织中，信噪比为 5.8。此外，所制备的双 Pdot 可以对淋巴管瓣膜和收缩频率进行成像。我们的研究为使用 Pdot 作为纳米探针进行代谢紊乱、骨骼系统疾病和循环系统等多尺度成像提供了一种可行的方法。
