Siboo R, Kulisek E
J Immunol Methods. 1978;23(1-2):59-67. doi: 10.1016/0022-1759(78)90109-6.
A sensitive fluorescent binding immunoassay (FBIA) was developed for measuring C-reactive protein (CRP), at a level of 20 ng/ml, in human and mouse sera. The advantages of the FBIA over radioimmunoassays are the long shelf-life of the FITC-labelled antibody, the re-use of the antibody bound microbeads, the short assay time and the use of inexpensive instruments.
已开发出一种灵敏的荧光结合免疫测定法（FBIA），用于检测人血清和小鼠血清中浓度为20 ng/ml的C反应蛋白（CRP）。与放射免疫测定法相比，FBIA的优势在于异硫氰酸荧光素（FITC）标记抗体的保质期长、抗体结合微珠可重复使用、测定时间短且使用的仪器成本低。
