SCORe：用于多重快速检测和假病毒中和的 SARS-CoV-2 奥密克戎变异 RBD 结合 DNA 适体。
SCORe: SARS-CoV-2 Omicron Variant RBD-Binding DNA Aptamer for Multiplexed Rapid Detection and Pseudovirus Neutralization.
机构信息
Department of Bioengineering, University of Washington, Seattle, Washington 98195, United States.
Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, United States.
出版信息
Anal Chem. 2022 Sep 20;94(37):12683-12690. doi: 10.1021/acs.analchem.2c01993. Epub 2022 Aug 16.
Abstract
During the COVID-19 (coronavirus disease 2019) pandemic, several SARS-CoV-2 variants of concern emerged, including the Omicron variant, which has enhanced infectivity and immune invasion. Many antibodies and aptamers that bind the spike (S) of previous strains of SARS-CoV-2 either do not bind or bind with low affinity to Omicron S. In this study, we report a high-affinity ARS-oV-2 micron BD-binding aptamr (SCORe) that binds Omicron BA.1 and BA.2 RBD with nanomolar . We employ aptamers SCORe.50 and SNAP4.74 in a multiplexed lateral flow assay (LFA) to distinguish between Omicron and wild-type S at concentrations as low as 100 pM. Finally, we show that SCORe.50 and its dimerized form SCOReD can neutralize Omicron S-pseudotyped virus infection of ACE2-overexpressing cells by >70%. SCORe therefore has potential applications in COVID-19 rapid diagnostics as well as in viral neutralization.
摘要
在 COVID-19（冠状病毒病 2019）大流行期间，出现了几种令人关注的 SARS-CoV-2 变体，包括具有增强传染性和免疫入侵能力的奥密克戎变体。许多与以前的 SARS-CoV-2 株的刺突（S）结合的抗体和适体要么不与奥密克戎 S 结合，要么结合亲和力低。在这项研究中，我们报告了一种高亲和力的 ARS-oV-2 微 BD 结合适体（SCORe），它以纳摩尔级亲和力结合奥密克戎 BA.1 和 BA.2 RBD。我们在多重侧向流动测定（LFA）中使用适体 SCORe.50 和 SNAP4.74 来区分奥密克戎和野生型 S，浓度低至 100 pM。最后，我们表明 SCORe.50 及其二聚体形式 SCOReD 可以中和 ACE2 过表达细胞中奥密克戎 S-假型病毒感染超过 70%。因此，SCORe 具有在 COVID-19 快速诊断以及病毒中和方面的潜在应用。
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