Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong, 226001, China.
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
J Cancer Res Clin Oncol. 2023 Jul;149(7):3649-3660. doi: 10.1007/s00432-022-04254-0. Epub 2022 Aug 16.
Lung cancer, the most prevalent cancer-related death worldwide, still lacks the means for early diagnosis. Because of the unique properties of the loop that make it stable in body fluids, circular RNAs (circRNAs) as a biomarker becomes a possibility. This research purposed to explore whether hsa_circ_0023179 can be applied as a possible biomarker for the early diagnosis and prognosis of non-small cell lung cancer (NSCLC).
hsa_circ_0023179 was screened by high-throughput sequencing of three pairs of NSCLC tissues and their surrounding tissues. Agarose gel electrophoresis (AGE), Sanger sequencing, exonuclease digestion assay, and actinomycin D were used to affirm the molecular properties of circRNA. Precision determination was performed by placement at room temperature and multiple freeze-thawing test for methodological evaluation. The expression of hsa_circ_0023179 in tissues, serum, and cells was determined by quantitative real-time polymerase chain reaction (qRT-PCR) to establish the receiver operating characteristic (ROC) curve to assess the diagnostic efficacy of hsa_circ_0023179.
hsa_circ_0023179 conforms to the basic properties of circRNA, and the detection method of hsa_circ_0023179 has good stability and repeatability. Its expression was connected to histological type, TNM stage, lymph node metastasis, and distal metastasis in NSCLC tissues, serum, and cells. Compared with traditional tumor markers with higher sensitivity and specificity. A combined diagnosis can significantly improve the diagnostic value. The decrease in postoperative expression level suggests some potential for dynamic monitoring.
hsa_circ_0023179 might be a promising novel serum marker for the detection and prediction of NSCLC.
肺癌是全球最常见的癌症相关死亡原因，但目前仍缺乏早期诊断的方法。由于环形 RNA（circRNA）的独特特性使其在体液中稳定存在，因此作为一种生物标志物成为可能。本研究旨在探讨 hsa_circ_0023179 是否可以作为非小细胞肺癌（NSCLC）早期诊断和预后的潜在生物标志物。
通过对三对 NSCLC 组织及其周围组织的高通量测序筛选 hsa_circ_0023179。采用琼脂糖凝胶电泳（AGE）、Sanger 测序、核酸外切酶消化实验和放线菌素 D 处理等方法验证 circRNA 的分子特性。通过室温放置和多次冻融实验进行方法学评估，对 circRNA 的检测方法进行精密度测定。通过定量实时聚合酶链反应（qRT-PCR）检测 hsa_circ_0023179 在组织、血清和细胞中的表达，构建受试者工作特征（ROC）曲线评估 hsa_circ_0023179 的诊断效能。
hsa_circ_0023179 符合 circRNA 的基本特性，hsa_circ_0023179 的检测方法具有良好的稳定性和重复性。其在 NSCLC 组织、血清和细胞中的表达与组织学类型、TNM 分期、淋巴结转移和远处转移有关。与传统肿瘤标志物相比，hsa_circ_0023179 具有更高的灵敏度和特异性。联合诊断可显著提高诊断价值。术后表达水平的降低提示其具有动态监测的潜力。
hsa_circ_0023179 可能是一种有前途的 NSCLC 检测和预测的新型血清标志物。
