儿童对 SARS-CoV-2 感染的体液和细胞反应的纵向研究。
Longitudinal survey of humoral and cellular response to SARS-CoV-2 infection in children.
机构信息
Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC) - Department of Pediatrics, Hospital Clínico San Carlos, C/Profesor Martín Lagos, SN, 28040 Madrid, Spain.
Department of Pediatrics, Hospital Clínico San Carlos, C/Profesor Martín Lagos, SN, 28040 Madrid, Spain.
出版信息
Microbiol Res. 2022 Nov;264:127145. doi: 10.1016/j.micres.2022.127145. Epub 2022 Jul 23.
BACKGROUND
Data regarding humoral and cellular response against SARS-CoV-2 in children are scarce. We analysed seroconversion rate, decrease of anti-RBD IgG antibodies over time and T-cell response in paediatric patients who suffered COVID-19.
METHODS
Longitudinal study of paediatric patients COVID-19 diagnosed by positive molecular assay in nasopharyngeal swabs. Blood samples were drawn 1-2 months and 6-7 months after acute infection. Anti-RBD IgG were determined using the Alinity® SARS-CoV-2 IgG II Quant assay (Abbott). Cellular immune response was analysed by T-SPOT® SARS-CoV-2 assay kit (Oxford Immunotec Ltd.).
RESULTS
27/39 (69,2%) patients seroconverted. Despite a significant decrease in antibody levels over time (p < 0,01), no children seroreverted between first and second visits. Only 6/16 (37,2%) children under 6 years-old were seropositive compared to 21/23 (91,3%) over 6 years-old (p < 0,01). Highest antibody levels were found in seropositive younger children (p = 0,036). Thirteen (33,3%) children showed T-cell response. Among participants showing humoral response, no cellular response was detected in 14 (51,9%).
CONCLUSIONS
Anti-RBD IgG antibodies persistence at 6-7-months after SARS-CoV-2 infection was observed. A different IgG response was found depending on age. As measured by T-SPOT, most patients did not display cellular response 6-7 months after infection.
背景
关于儿童对 SARS-CoV-2 的体液和细胞反应的数据很少。我们分析了患有 COVID-19 的儿科患者的血清转化率、抗 RBD IgG 抗体随时间的下降以及 T 细胞反应。
方法
对经鼻咽拭子分子检测确诊为 COVID-19 的儿科患者进行纵向研究。在急性感染后 1-2 个月和 6-7 个月抽取血液样本。使用 Alinity® SARS-CoV-2 IgG II Quant 测定法（雅培）测定抗 RBD IgG。通过 T-SPOT® SARS-CoV-2 检测试剂盒（Oxford Immunotec Ltd.）分析细胞免疫反应。
结果
39 例患者中的 27 例（69.2%）发生血清转化。尽管抗体水平随时间显著下降（p<0.01），但在首次和第二次就诊之间没有儿童出现血清学逆转。与 6 岁以上的 21/23 例（91.3%）相比，6 岁以下的 16 例儿童中只有 6 例（37.2%）呈阳性（p<0.01）。阳性的年幼儿童抗体水平最高（p=0.036）。13 例（33.3%）儿童出现 T 细胞反应。在显示体液反应的参与者中，14 例（51.9%）未检测到细胞反应。
结论
在 SARS-CoV-2 感染后 6-7 个月观察到抗 RBD IgG 抗体的持续存在。根据年龄发现了不同的 IgG 反应。通过 T-SPOT 测量，大多数患者在感染后 6-7 个月未显示细胞反应。
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