Yang Yuanxue, Duan Ailing, Zhang Cong, Zhang Yun, Wang Aiyu, Xue Chao, Wang Hongyan, Zhao Ming, Zhang Jianhua
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Department of Plant Protection, Shandong Agricultural University, Tai'an 271018, China.
Pestic Biochem Physiol. 2022 Aug;186:105170. doi: 10.1016/j.pestbp.2022.105170. Epub 2022 Jul 8.
Aphis craccivora (Koch), a globally pest that causes significant threat to the legumes, has developed different degrees of resistance to a variety of insecticides. The ATP-binding cassette (ABC) transporters comprise a multifunctional transporter protein superfamily which play important roles in the transport and detoxification of xenobiotic compounds in insects. However, whether ABC transporters take part in the tolerance of imidacloprid in A. craccivora is still unknown. In order to investigate the functions of ABC transporters in the imidacloprid tolerance, fifty- eight ABC transporters were identified in the transcriptome and genome of A. craccivora and the toxicity of imidacloprid against A. craccivora was significantly increased after application the inhibitors of verapamil and Ko143. The relative expression levels of ABCG5, ABCG6, ABCG10, ABCH3, ABCH4, ABCH8 and ABCH10 were significantly up-regulated in response to imidacloprid treatment with LC, LC and LC concentrations, and the expression patterns of these seven ABC transporters were further analyzed at different developmental stages and in different tissues of A. craccivora by quantitative real-time PCR (RT-qPCR). Furthermore, knockdown of ABCG10, ABCH3 and ABCH4 significantly increased the mortality of A. craccivora to imidacloprid. Our results reveal the key functions of ABC transporters in the tolerance of imidacloprid and provide valuable information regarding the development of improved management strategies in A. craccivora.
豆蚜（Aphis craccivora (Koch)）是一种对全球豆类作物造成重大威胁的害虫，已对多种杀虫剂产生了不同程度的抗性。ATP结合盒（ABC）转运蛋白构成了一个多功能转运蛋白超家族，在昆虫对外源化合物的转运和解毒过程中发挥着重要作用。然而，ABC转运蛋白是否参与豆蚜对吡虫啉的耐受性仍不清楚。为了研究ABC转运蛋白在吡虫啉耐受性中的功能，在豆蚜的转录组和基因组中鉴定出了58个ABC转运蛋白，并且在施用维拉帕米和Ko143抑制剂后，吡虫啉对豆蚜的毒性显著增加。在LC、LC和LC浓度的吡虫啉处理下，ABCG5、ABCG6、ABCG10、ABCH3、ABCH4、ABCH8和ABCH10的相对表达水平显著上调，并且通过定量实时PCR（RT-qPCR）进一步分析了这7个ABC转运蛋白在豆蚜不同发育阶段和不同组织中的表达模式。此外，敲低ABCG10、ABCH3和ABCH4显著增加了豆蚜对吡虫啉的死亡率。我们的研究结果揭示了ABC转运蛋白在吡虫啉耐受性中的关键功能，并为制定改进的豆蚜管理策略提供了有价值的信息。
