Holmes R E, Hagler H K, Coletta C A
J Biomed Mater Res. 1987 Jun;21(6):731-9. doi: 10.1002/jbm.820210605.
A histometry system has been developed to measure bone ingrowth into porous hydroxyapatite implants utilizing the backscattered electron image of thick sections. The system consists of a scanning electron microscope (SEM) with backscattered electron detector, digital beam controller, minicomputer based image digitization, and microcomputer based image processing, point counting, and lineal analysis. The SEM backscattered electron imaging mode yields high tissue contrast and sharp tissue boundaries, substantially reducing the lost cap and projection effect errors of thick sections. High-resolution digitization of the image substantially reduces the standard error of the estimates. By using the digitized image the tedious process of filtering artifacts and recording actual point counts, intersections, and intercept lengths is delegated to computer software. Performance of this system in a recent study demonstrated substantial ease of operator use and speed of measurement. In the absence of a digital beam controller an inexpensive video digitizer circuit board may be used to digitize photographic prints of the SEM images. The combination of increased accuracy, precision, operator ease, and speed suggests that this system can be useful for soft tissue-bone-implant histometry.
已开发出一种组织测量系统，用于利用厚切片的背散射电子图像来测量骨长入多孔羟基磷灰石植入物的情况。该系统由一台配备背散射电子探测器的扫描电子显微镜（SEM）、数字束控制器、基于小型计算机的图像数字化以及基于微型计算机的图像处理、点计数和线性分析组成。SEM背散射电子成像模式可产生高组织对比度和清晰的组织边界，大大减少了厚切片的帽状丢失和投影效应误差。图像的高分辨率数字化大大降低了估计值的标准误差。通过使用数字化图像，过滤伪像以及记录实际点计数、交点和截距长度的繁琐过程可交给计算机软件处理。该系统在最近一项研究中的表现表明，其操作简便且测量速度快。在没有数字束控制器的情况下，可使用廉价的视频数字化电路板对SEM图像的照片打印件进行数字化。更高的准确性、精度、操作简便性和速度表明，该系统可用于软组织-骨-植入物组织测量。
