中国西南地区炎症性肠病患者肠道菌群的差异
Differences in the Intestinal Flora of Patients with Inflammatory Bowel Disease in Southwest China.
作者信息
Wang Tingting, Yu Renlin, Zhu Lingling, Wang Xuean, Yang Bin
机构信息
Department of Laboratory Medicine, West China Hospital of Sichuan University, Chengdu, China.
Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
出版信息
Indian J Microbiol. 2022 Sep;62(3):384-392. doi: 10.1007/s12088-022-01014-z. Epub 2022 Mar 24.
UNLABELLED
To study changes in the intestinal flora associated with inflammatory bowel disease (IBD) in the Han population of southwest China, 48 participants were enrolled, 18 of whom had been diagnosed with IBD. Stool samples were collected from the participants. Sequencing of 16S rRNA gene was used to measure and identify the components of the intestinal flora. Diversity analysis and multivariate statistical analysis were conducted to study differences in intestinal flora between patients with IBD and healthy controls. The goods coverage, observed species, Shannon, and Simpson indices of alpha diversity were different ( < 0.05). Beta diversity analysis yielded significant differences between groups (R = 0.5668, = 0.001 < 0.05). Compared with the composition of the intestinal flora in healthy controls, the relative abundances of (18.56% vs. 3.56%, = 0.001) and (2.08% vs. 0.35%, = 0.005) were higher in patients with IBD. Therefore, this study provides insight into the role of the microbiome in IBD.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12088-022-01014-z.
未标注
为研究中国西南地区汉族人群中与炎症性肠病（IBD）相关的肠道菌群变化，招募了48名参与者，其中18人被诊断为IBD。从参与者中采集粪便样本。使用16S rRNA基因测序来测量和鉴定肠道菌群的组成部分。进行多样性分析和多变量统计分析以研究IBD患者与健康对照者肠道菌群的差异。α多样性的覆盖率、观测物种数、香农指数和辛普森指数均存在差异（P＜0.05）。β多样性分析显示组间存在显著差异（R = 0.5668，P = 0.001＜0.05）。与健康对照者的肠道菌群组成相比，IBD患者中[具体菌属1]（18.56%对3.56%，P = 0.001）和[具体菌属2]（2.08%对0.35%，P = 0.005）的相对丰度更高。因此，本研究为微生物群在IBD中的作用提供了见解。
补充信息
在线版本包含可在10.1007/s12088-022-01014-z获取的补充材料。
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