Ma Jianxia, Wang Ke, Wang Jun, Zeng Qinlian, Liu Kangwei, Zheng Songbai, Chen Yuanwen, Yao Jianfeng
Department of Gastroenterology, Hua Dong Hospital of Fu Dan University, Shanghai, 200040, People's Republic of China.
Int J Gen Med. 2024 Apr 6;17:1355-1367. doi: 10.2147/IJGM.S448359. eCollection 2024.
The fecal microbiota was studied in patients with inflammatory bowel disease (IBD), and the characteristics of gut microbiota were compared among patients with different subtypes and stages of IBD, aiming to identify the gut microbiota associated with IBD.
Fecal samples were collected from 41 IBD patients (18 patients with ulcerative colitis [UC] and 23 patients with Crohn's disease [CD]) in the Department of Gastroenterology of East China Hospital, Fudan University between January 2021 and January 2022. In addition, fecal samples were collected from 20 healthy volunteers. The fecal microbiota was subjected to 16S rRNA gene sequencing, followed by bioinformatics analysis.
There was significant difference in the fecal microbiota between IBD patients and controls. The abundance and diversity of fecal microbiota in the IBD patients were significantly lower than in controls. The relative abundance of and was reduced markedly in the IBD patients. As compared to controls, the relative abundance of increased dramatically in the UC patients. The relative abundance of and significantly increased in the CD patients. As compared to CD patients, the relative abundance of was reduced markedly in the UC patients; the relative abundance of and was reduced dramatically in the CD patients. In addition, significant difference was also noted in the fecal microflora between patients with active IBD and those with IBD in remission period. In active IBD patients, the relative abundance of and was reduced significantly.
There is intestinal microbiota imbalance in IBD patients, and the abundance of and is reduced significantly in the active period of IBD, which may be related to the active IBD.
对炎症性肠病（IBD）患者的粪便微生物群进行研究，比较不同亚型和阶段的IBD患者肠道微生物群的特征，旨在识别与IBD相关的肠道微生物群。
2021年1月至2022年1月期间，从复旦大学附属华东医院消化内科的41例IBD患者（18例溃疡性结肠炎[UC]患者和23例克罗恩病[CD]患者）中收集粪便样本。此外，还从20名健康志愿者中收集粪便样本。对粪便微生物群进行16S rRNA基因测序，随后进行生物信息学分析。
IBD患者与对照组的粪便微生物群存在显著差异。IBD患者粪便微生物群的丰度和多样性显著低于对照组。IBD患者中[具体菌种1]和[具体菌种2]的相对丰度明显降低。与对照组相比，UC患者中[具体菌种3]的相对丰度显著增加。CD患者中[具体菌种4]和[具体菌种5]的相对丰度显著增加。与CD患者相比，UC患者中[具体菌种6]的相对丰度明显降低；CD患者中[具体菌种7]和[具体菌种8]的相对丰度显著降低。此外，活动期IBD患者与缓解期IBD患者的粪便微生物群也存在显著差异。在活动期IBD患者中，[具体菌种9]和[具体菌种10]的相对丰度显著降低。
IBD患者存在肠道微生物群失衡，且在IBD活动期[具体菌种11]和[具体菌种12]的丰度显著降低，这可能与IBD的活动有关。
