Hakiminia Saeed, Esmaeeli Zahra, Moghadamnia Ali Akbar, Jorsaraei Seyyed Gholam Ali, Feizi Farideh, Khafri Sorayya, Memariani Zahra, Shirafkan Hoda, Mozaffarpur Seyyed Ali
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Department of Pharmacology, Babol University of Medical Sciences, Babol, Iran.
Caspian J Intern Med. 2022 Summer;13(3):475-483. doi: 10.22088/cjim.13.3.475.
was used traditionally as laxative in pregnant women. Nevertheless, its fetal and maternal effects in pregnancy have not been studied yet.
Oral (Lethal Dose, 50%) LD50 was determined in mice. In addition, a control group, pregnant rats in other 5 experimental groups (n=12) received orally aqueous extract (500, 1000 and 2000 mg/kg), tween80 (10%) and distilled water during pregnancy up to the delivery (21-23 days). Some serum indices were evaluated in maternal blood samples after delivery. Histopathologic and histomorphometric evaluations were performed on the selected slices of newborn rats.
Anthraquinone content of the aqueous extract was 0.34% w/w. Oral LD50 was obtained more than 5000mg/kg. No abortions and newborn anomalies were observed in groups. The height and weight of the offspring were significantly reduced by the administration of 500, and 2000 mg/kg of extract compared to control. There was no significant change in maternal blood urea and creatinine. Higher concentration (2000mg/kg) led to ALT elevation. ALS levels decreased dose-dependency in treatment groups comparing to control. Histopathological findings showed significant lung vascular congestion, and hypertrophy of heart in group tween80, and significant hepatic parenchymal inflammation in tween80 and 2000mg/kg and 1000mg/kg groups. In all tissues of all groups, malpighian body area and bowman's capsule space significantly increased compared to the control group.
It seems extract is safe in pregnancy. Because of confounding role of tween80 in histopathological finding, more research is necessary.
传统上被用作孕妇的泻药。然而，其在孕期对胎儿和母亲的影响尚未得到研究。
测定小鼠口服半数致死量（LD50）。此外，设立一个对照组，其他5个实验组（n = 12）的怀孕大鼠在孕期直至分娩（21 - 23天）口服水提取物（500、1000和2000毫克/千克）、吐温80（10%）和蒸馏水。分娩后对母体血液样本中的一些血清指标进行评估。对新生大鼠的选定切片进行组织病理学和组织形态计量学评估。
水提取物的蒽醌含量为0.34% w/w。口服LD50超过5000毫克/千克。各实验组均未观察到流产和新生仔异常。与对照组相比，给予500毫克/千克和2000毫克/千克提取物使后代的身高和体重显著降低。母体血尿素和肌酐无显著变化。较高浓度（2000毫克/千克）导致谷丙转氨酶升高。与对照组相比，治疗组的碱性磷酸酶水平呈剂量依赖性降低。组织病理学结果显示，吐温80组有明显的肺血管充血和心脏肥大，吐温80组、2000毫克/千克组和1000毫克/千克组有明显的肝实质炎症。与对照组相比，所有组所有组织的马尔皮基氏体面积和鲍曼囊间隙均显著增加。
看来该提取物在孕期是安全的。由于吐温80在组织病理学结果中的混杂作用，有必要进行更多研究。
