Translational Mass Spectrometry Research Group, Genetics & Genomic Medicine Department, UCL Institute of Child Health, London, UK.
Great Ormond Street Biomedical Research Centre, UCL Institute of Child Health London.
Cell Rep Methods. 2022 Sep 19;2(9):100279. doi: 10.1016/j.crmeth.2022.100279. Epub 2022 Aug 12.
Determining the protection an individual has to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants of concern (VoCs) is crucial for future immune surveillance, vaccine development, and understanding of the changing immune response. We devised an informative assay to current ELISA-based serology using multiplexed, baited, targeted proteomics for direct detection of multiple proteins in the SARS-CoV-2 anti-spike antibody immunocomplex. Serum from individuals collected after infection or first- and second-dose vaccination demonstrates this approach and shows concordance with existing serology and neutralization. Our assays show altered responses of both immunoglobulins and complement to the Alpha (B.1.1.7), Beta (B.1.351), and Delta (B.1.617.1) VoCs and a reduced response to Omicron (B1.1.1529). We were able to identify individuals who had prior infection, and observed that C1q is closely associated with IgG1 (r > 0.82) and may better reflect neutralization to VoCs. Analyzing additional immunoproteins beyond immunoglobulin (Ig) G, provides important information about our understanding of the response to infection and vaccination.
确定个体对严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）变体（VOCs）的保护程度对于未来的免疫监测、疫苗开发以及对不断变化的免疫反应的理解至关重要。我们设计了一种信息丰富的分析方法，该方法基于当前的 ELISA 血清学，使用多重、诱饵、靶向蛋白质组学直接检测 SARS-CoV-2 抗刺突抗体免疫复合物中的多种蛋白质。从感染后或第一剂和第二剂疫苗接种后收集的个体的血清证明了这种方法，并与现有的血清学和中和作用一致。我们的检测表明，针对 Alpha（B.1.1.7）、Beta（B.1.351）和 Delta（B.1.617.1）VOCs 的免疫球蛋白和补体的反应发生了改变，并且对 Omicron（B1.1.1529）的反应降低。我们能够识别出先前感染过的个体，并观察到 C1q 与 IgG1 密切相关（r>0.82），并且可能更好地反映对 VOCs 的中和作用。分析免疫球蛋白（Ig）G 以外的其他免疫蛋白提供了有关我们对感染和疫苗接种反应的理解的重要信息。
