Department of Digestive Internal Medicine, Seventh People's Hospital of Shanghai University of TCM, 358 Gaoqiao Datong Road, Shanghai, 200137, China.
Clin Exp Med. 2023 Sep;23(5):1633-1639. doi: 10.1007/s10238-022-00870-9. Epub 2022 Aug 17.
Numerous pieces of evidence have demonstrated the functional role of miR-548 in various cancers. The expression and function of miR-548 in gastric cancer were investigated in the present study. A total of 123 gastric cancer patients were included and provided paired gastric cancer tissues and matched normal tissues. RT-qPCR was used to detect the expression of miR-548. CCK8 assay was used to evaluate cell proliferation, and Transwell assay was applied to assess cell migration and invasion. The clinical significance of miR-548 was estimated by a series of statistical analyses. miR-548 was found to be upregulated in gastric cancer, which was associated with the lymph node metastasis and TNM stage of patients. Patients with relatively high miR-548 expression possessed bad survival. miR-548 was identified as a prognostic indicator of gastric cancer. miR-548 was also found to promote the proliferation, migration, and invasion of gastric cancer. Upregulated miR-548 was involved in the progression of gastric cancer and predicted the prognosis of patients. Inhibition of miR-548 might be a novel therapeutic strategy for gastric cancer.
大量证据表明 miR-548 在多种癌症中具有功能作用。本研究旨在探讨 miR-548 在胃癌中的表达和功能。共纳入 123 例胃癌患者，提供配对的胃癌组织和匹配的正常组织。采用 RT-qPCR 检测 miR-548 的表达。CCK8 检测细胞增殖，Transwell 检测细胞迁移和侵袭。通过一系列统计学分析评估 miR-548 的临床意义。研究发现 miR-548 在胃癌中上调，与患者的淋巴结转移和 TNM 分期相关。miR-548 表达较高的患者生存状况较差。miR-548 被确定为胃癌的预后指标。miR-548 还促进了胃癌的增殖、迁移和侵袭。上调的 miR-548 参与了胃癌的进展，并预测了患者的预后。抑制 miR-548 可能成为胃癌的一种新的治疗策略。
