Dental student, Department of Oral and Maxillofacial Medicine, School of Dentistry, Hamadan University of Medical sciences, Hamadan, Iran.
Assistant Professor, Department of Oral and Maxillofacial Medicine, School of Dentistry, Hamadan University of Medical sciences, Hamadan, Iran.
Rom J Intern Med. 2022 Nov 23;60(4):215-221. doi: 10.2478/rjim-2022-0013. Print 2022 Dec 1.
Obesity is a worldwide concern that may lead to type 2 diabetes, cardiovascular diseases, etc. Several serum biomarkers have been identified in the saliva of obese individuals, including inflammatory cytokines, adipokines, insulin, and cortisol. The present study aimed to compare salivary interleukin-6 (IL-6), interleukin-8 (IL-8), C-reactive protein (CRP) levels and total antioxidants capacity (TAC) of obese individuals with normal-weighted ones. In this case-control study, 92 participants matched in terms of age and gender were placed into two groups according to the body mass index (BMI); case group: BMI>30 and control group: 18.5<BMI<24.99. Unstimulated saliva was collected. ELISA and FRAP method were used to determine IL-6, IL-8, CRP and TAC. Data was analyzed using SPSS 24, at the significant level of 0.05. Mean salivary levels of IL-6 and IL-8 in the normal individuals were 53.36 and 421.25ng/mL, with 86.09 ng/mL and 510.19 ng/mL in obese individuals, respectively. There were significant differences in the mean salivary levels of IL-6 and IL-8 between two experimental groups. The mean salivary levels of CRP of control and case group was 2.84 and 2.63 ng/mL and the total salivary antioxidant levels in the normal and obese individuals were 0.29 and 0.36, respectively which had no significant different. According to the results of the present study, salivary levels IL-8 and IL-6 was significantly higher in obese individuals than in those with normal weight. However, the mean salivary CRP and TAC were not significantly different between the obese individuals and normal-weighted ones.
肥胖是一个全球性的问题，可能导致 2 型糖尿病、心血管疾病等。已经在肥胖个体的唾液中鉴定出几种血清生物标志物，包括炎症细胞因子、脂肪因子、胰岛素和皮质醇。本研究旨在比较肥胖个体和正常体重个体的唾液白细胞介素-6（IL-6）、白细胞介素-8（IL-8）、C 反应蛋白（CRP）水平和总抗氧化能力（TAC）。在这项病例对照研究中，根据体重指数（BMI）将 92 名年龄和性别匹配的参与者分为两组；病例组：BMI>30；对照组：18.5<BMI<24.99。收集非刺激唾液。使用 ELISA 和 FRAP 法测定 IL-6、IL-8、CRP 和 TAC。使用 SPSS 24 进行数据分析，显著性水平为 0.05。正常个体唾液中 IL-6 和 IL-8 的平均水平分别为 53.36 和 421.25ng/mL，肥胖个体分别为 86.09 和 510.19ng/mL。两组实验中 IL-6 和 IL-8 的唾液平均水平存在显著差异。对照组和病例组 CRP 的唾液平均水平分别为 2.84 和 2.63ng/mL，正常和肥胖个体的总唾液抗氧化水平分别为 0.29 和 0.36，无显著差异。根据本研究的结果，肥胖个体的唾液 IL-8 和 IL-6 水平明显高于正常体重个体。然而，肥胖个体和正常体重个体之间的 CRP 和 TAC 平均唾液水平没有显著差异。
