Functional fiber enhances the effect of every-other-day fasting on insulin sensitivity by regulating the gut microecosystem.

Every-other-day fasting (EODF), which involves alternating days of fasting and feeding, has been reported to lower obesity, and dietary fibers can improve metabolism by altering gut microbiota. This study investigated whether the combination of functional fiber (FF) and EODF (FF-EODF) can further improve insulin sensitivity by regulating the composition of microbiota and curbing weight gain. Twenty-eight diet-induced obese (DIO) mice were randomly divided into four experimental groups (n=7): (1) ad-libitum (AL), (2) EODF, (3) 4% FF-EODF and (4) 6% FF-EODF. After exposure to a high-fat basal diet (HFD) for 12 weeks (1-12 weeks, period 1) and then to a normal chow diet (NCD) for 4 weeks (13-16 weeks, period 2). Compared with EODF alone, 6% FF-EODF treatment could significantly improve the insulin sensitivity of DIO mice without affecting their body weight during period 1(HFD), while significantly increasing satiety, energy consumption, weight, and adipose loss, and insulin sensitivity during period 2 (NCD). Meanwhile, FF-EODF showed a higher increase in short-chain fatty acids (SCFAs) and restored the proportion of induced intraepithelial lymphocytes in the intestinal epithelium compared to EODF alone. Although EODF could increase the relative abundances of Lactobacillus and Bifidobacteriumin, FF supplementation further increased the relative abundance of Lactobacillus, Bifidobacterium, and S24-7 in the intestine. This increase was positively correlated with the decrease in adiposity and insulin resistance, indicating that FF plays a key role in insulin improvement. Our study demonstrated the potential of FF-EODF in promoting insulin sensitivity and reducing body weight via beneficial regulation of gut microecosystem.