School of Biological and Food Engineering, Suzhou University, Suzhou, 234000 Anhui, China.
Biomed Res Int. 2022 Aug 8;2022:6293095. doi: 10.1155/2022/6293095. eCollection 2022.
With the continuous improvement of human living standards, people's demand for health has become an important international research hotspot. In recent years, 41.3% of the total incidence of multiple organ failure (MOF) caused by dysfunction of the intestinal screen was found every year. The mortality rate is 62%, which is more than twice that of developed countries. This paper is aimed at observing the microscopic effects of probiotics derived from dairy products using Caco-2 cells on intestinal barrier function. Based on the above background, the purpose of this study was to construct a Caco-2 cell model under microscope to study the effect of probiotics on intestinal barrier function. This study first describes the background knowledge of the integration of modern microscope technology and medical field and the correlation between them. The results showed that the relative adhesion rates of Lactobacillus bulgaricus, Lactobacillus acidophilus, and Streptococcus thermophilus were 4.67 ± 0.07%, 11.53 ± 0.06%, and 18.31 ± 0.08%, respectively, which were lower than those in the normal group. The production of antibacterial substances can inhibit intestinal pathogens and adjust the balance of intestinal flora.
随着人类生活水平的不断提高，人们对健康的需求已成为国际重要的研究热点。近年来，每年因肠道屏障功能障碍导致多器官功能衰竭（MOF）的总发病率中，有 41.3%是由肠道屏障功能障碍引起的。死亡率为 62%，是发达国家的两倍多。本文旨在观察乳制品来源的益生菌对 Caco-2 细胞肠屏障功能的微观影响。基于上述背景，本研究旨在构建显微镜下的 Caco-2 细胞模型，研究益生菌对肠屏障功能的影响。本研究首先描述了现代显微镜技术与医学领域融合的背景知识及其相关性。结果表明，保加利亚乳杆菌、嗜酸乳杆菌和嗜热链球菌的相对黏附率分别为 4.67±0.07%、11.53±0.06%和 18.31±0.08%，均低于正常组。抗菌物质的产生可以抑制肠道病原体，调节肠道菌群平衡。
