Radzieta Michael, Malone Matthew, Ahmad Mehtab, Dickson Hugh G, Schwarzer Saskia, Jensen Slade O, Lavery Lawrence A
South West Sydney Limb Preservation and Wound Research, South Western Sydney Local Health District (LHD), Sydney, NSW, Australia.
Infectious Diseases and Microbiology, School of Medicine, Western Sydney University, Sydney, NSW, Australia.
Front Microbiol. 2022 Aug 1;13:956332. doi: 10.3389/fmicb.2022.956332. eCollection 2022.
Osteomyelitis in the feet of persons with diabetes is clinically challenging and is associated with high rates of amputation. In this study RNA-sequencing was employed to explore microbial metatranscriptomes with a view to understand the relative activity and functions of the pathogen/s responsible for diabetes foot osteomyelitis (DFO). We obtained 25 intraoperative bone specimens from persons with confirmed DFO, observing that spp. (7%), spp. (7%), spp. (6%), spp. (5%) and spp. (5%) are the most active taxa on average. Data was then subset to examine functions associated with pathogenesis (virulence and toxins), biofilm formation and antimicrobial/multi-drug resistance. Analysis revealed spp. are the most active taxa relative to pathogenic functions with K06218 (mRNA interferase ), K03699 (membrane damaging toxin ) and K03980 (putative peptidoglycan lipid II flippase ), K01114 (membrane damaging toxin plc) and K19168 (toxin cptA) being the most prevalent pathogenic associated transcripts. The most abundant transcripts associated with biofilm pathways included components of the biofilm EPS matrix including glycogen synthesis, cellulose synthesis, colonic acid synthesis and flagella synthesis. We further observed enrichment of a key enzyme involved in the biosynthesis of L-rhamnose (K01710 -dTDP-glucose 4,6-dehydratase ) which was present in all but four patients with DFO.
糖尿病患者足部骨髓炎在临床上具有挑战性，且与高截肢率相关。在本研究中，采用RNA测序来探索微生物元转录组，以了解导致糖尿病足骨髓炎（DFO）的病原体的相对活性和功能。我们从确诊为DFO的患者身上获取了25份术中骨标本，观察到平均而言， 属（7%）、 属（7%）、 属（6%）、 属（5%）和 属（5%）是最活跃的分类群。然后对数据进行子集分析，以检查与发病机制（毒力和毒素）、生物膜形成以及抗菌/多药耐药性相关的功能。分析显示，相对于致病功能而言， 属是最活跃的分类群，其中K06218（mRNA干扰酶 ）、K03699（膜损伤毒素 ）和K03980（假定的肽聚糖脂质II翻转酶 ）、K01114（膜损伤毒素plc）和K19168（毒素cptA）是最普遍的致病相关转录本。与生物膜途径相关的最丰富转录本包括生物膜EPS基质的成分，包括糖原合成、纤维素合成、结肠酸合成和鞭毛合成。我们进一步观察到参与L - 鼠李糖生物合成的一种关键酶（K01710 - dTDP - 葡萄糖4,6 - 脱水酶 ）的富集，除了4例DFO患者外，该酶在所有患者中均存在。
