Department of Biology, School of Life Sciences, Southern University of Science and Technology, Shenzhen, China.
Institute of Plant and Food Science, Southern University of Science and Technology, Shenzhen, China.
Nat Plants. 2022 Sep;8(9):1118-1126. doi: 10.1038/s41477-022-01224-9. Epub 2022 Aug 18.
Poly(A) tail is a hallmark of eukaryotic messenger RNA and its length plays an essential role in regulating mRNA metabolism. However, a comprehensive resource for plant poly(A) tail length has yet to be established. Here, we applied a poly(A)-enrichment-free, nanopore-based method to profile full-length RNA with poly(A) tail information in plants. Our atlas contains over 120 million polyadenylated mRNA molecules from seven different tissues of Arabidopsis, as well as the shoot tissue of maize, soybean and rice. In most tissues, the size of plant poly(A) tails shows peaks at approximately 20 and 45 nucleotides, while the poly(A) tails in pollen exhibit a distinct pattern with strong peaks centred at 55 and 80 nucleotides. Moreover, poly(A) tail length is regulated in a gene-specific manner-mRNAs with short half-lives in general have long poly(A) tails, while mRNAs with long half-lives are featured with relatively short poly(A) tails that peak at ~45 nucleotides. Across species, poly(A) tails in the nucleus are almost twice as long as in the cytoplasm. Our comprehensive dataset lays the groundwork for future functional and evolutionary studies on poly(A) tail length regulation in plants.
多聚（A）尾是真核信使 RNA 的标志，其长度在调节 mRNA 代谢中起着至关重要的作用。然而，植物多聚（A）尾长度的综合资源尚未建立。在这里，我们应用了一种无多聚（A）尾巴富集的基于纳米孔的方法，来描绘植物中具有多聚（A）尾巴信息的全长 RNA。我们的图谱包含了来自拟南芥的七个不同组织以及玉米、大豆和水稻的茎组织的超过 1.2 亿个多聚腺苷酸化的 mRNA 分子。在大多数组织中，植物多聚（A）尾巴的大小在大约 20 和 45 个核苷酸处显示出峰值，而花粉中的多聚（A）尾巴则呈现出明显的模式，其中心在 55 和 80 个核苷酸处有很强的峰值。此外，多聚（A）尾巴的长度以基因特异性的方式进行调节——一般半衰期较短的 mRNAs 具有较长的多聚（A）尾巴，而半衰期较长的 mRNAs 则具有相对较短的多聚（A）尾巴，其峰值在大约 45 个核苷酸处。在不同的物种中，细胞核中的多聚（A）尾巴几乎是细胞质中的两倍长。我们的综合数据集为未来在植物中研究多聚（A）尾巴长度调节的功能和进化研究奠定了基础。
