Samim Abdur Rouf, Singh Vinay Kumar, Vaseem Huma
Department of Zoology, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, India.
Department of Zoology, CMP Degree College, University of Allahabad, Prayagraj 211002, India.
J Trace Elem Med Biol. 2022 Dec;74:127059. doi: 10.1016/j.jtemb.2022.127059. Epub 2022 Aug 4.
The aim of the present study was to assess the hazardous impact of nickel oxide nanoparticles (NiO NPs) on gills and liver of Heteropneustes fossilis.
Fishes were treated with four concentrations of NiO NPs for a period of 14 days. Nickel accumulation, lipid peroxidation, antioxidant enzymes activities (superoxide dismutase, catalase, glutathione s transferase & glutathione reductase), liver enzymes activities (aspartate amino transferase, alanine transaminase, & alkaline phosphatase), Na/K ATPase activity, FTIR, metallothionein content, ethoxyresorufin-o-deethylase activity, immunohistochemistry, histology and scanning electron microscopy were analyzed in both gills and liver tissues.
Results revealed increased accumulation of nickel in both the tissues of exposed fishes. Lipid peroxidation and activities of different antioxidant enzymes increased (except superoxide dismutase) in both the tissues after exposure. Fluctuations in liver enzymes activities and variation in the activity of Na/K ATPase were also observed. FTIR data revealed shift in peaks position in both the tissues. Level of metallothionein and its expression as well as activity of ethoxyresorufin-o-deethylase and expression of CYP1A also increased in both the target tissues of treated fishes. Furthermore, histological investigation and scanning electron microscopy showed structural damages in gills as well as liver tissues of exposed fishes.
Our results suggest that NiO NPs cause deteriorating effects on the gill and liver tissues of fish, therefore effluents containing these nanoparticles should be treated before their release into water bodies.
本研究的目的是评估氧化镍纳米颗粒（NiO NPs）对印度鲶鱼鳃和肝脏的有害影响。
用四种浓度的NiO NPs处理鱼类14天。分析了鳃和肝脏组织中的镍积累、脂质过氧化、抗氧化酶活性（超氧化物歧化酶、过氧化氢酶、谷胱甘肽S转移酶和谷胱甘肽还原酶）、肝脏酶活性（天冬氨酸氨基转移酶、丙氨酸氨基转移酶和碱性磷酸酶）、钠钾ATP酶活性、傅里叶变换红外光谱、金属硫蛋白含量、乙氧基异吩唑酮 - O - 脱乙基酶活性、免疫组织化学、组织学和扫描电子显微镜。
结果显示，暴露鱼类的两个组织中镍积累增加。暴露后，两个组织中的脂质过氧化和不同抗氧化酶的活性均增加（超氧化物歧化酶除外）。还观察到肝脏酶活性的波动和钠钾ATP酶活性的变化。傅里叶变换红外光谱数据显示两个组织中的峰位发生了偏移。处理鱼类的两个靶组织中金属硫蛋白水平及其表达以及乙氧基异吩唑酮 - O - 脱乙基酶活性和CYP1A表达也增加。此外，组织学研究和扫描电子显微镜显示暴露鱼类的鳃和肝脏组织有结构损伤。
我们的结果表明，NiO NPs对鱼类的鳃和肝脏组织有恶化作用，因此含有这些纳米颗粒的废水在排放到水体之前应进行处理。
