Grogan C K, Kim H K, Romsos D R
J Nutr. 1987 Jun;117(6):1115-20. doi: 10.1093/jn/117.6.1115.
We reported previously that adrenalectomy reduced the energy density of body weight gain (an indicator of proportional gain in lean and fat tissue) and the efficiency of energy retention in obese (ob/ob) mice to values approximating those in lean mice, but that adrenalectomy had much less influence on these parameters in ob/ob mice fed a purified high fat diet. To determine if fat was the exclusive factor in the purified high fat diet that negated effects of adrenalectomy, ob/ob mice were fed a purified high carbohydrate (glucose) diet identical in composition to the high fat diet, except for the fat/carbohydrate ratio. Responses of adrenalectomized ob/ob mice fed the purified high glucose diet from 4 to 7 wk of age mimicked those of mice fed the purified high fat diet, not those of mice fed the high carbohydrate nonpurified diet. Plasma glucose responses to a glucose load in adrenalectomized ob/ob mice paralleled the diet-dependent changes in energy balance. These results demonstrate that diet composition interacts with adrenal secretions to influence energy and glucose metabolism in ob/ob mice; consumption of either a purified high glucose or high fat diet negates the beneficial effects of adrenalectomy on energy and glucose metabolism observed when adrenalectomized ob/ob mice consume a nonpurified diet.
我们之前报道过，肾上腺切除术可降低肥胖（ob/ob）小鼠体重增加的能量密度（瘦组织和脂肪组织比例增加的指标）以及能量保留效率，使其接近瘦小鼠的水平，但肾上腺切除术对喂食纯化高脂饮食的ob/ob小鼠的这些参数影响较小。为了确定脂肪是否是纯化高脂饮食中抵消肾上腺切除术作用的唯一因素，给ob/ob小鼠喂食一种纯化高碳水化合物（葡萄糖）饮食，其成分与高脂饮食相同，只是脂肪/碳水化合物比例不同。4至7周龄时喂食纯化高葡萄糖饮食的肾上腺切除ob/ob小鼠的反应与喂食纯化高脂饮食的小鼠相似，而不是与喂食高碳水化合物非纯化饮食的小鼠相似。肾上腺切除ob/ob小鼠对葡萄糖负荷的血浆葡萄糖反应与饮食依赖的能量平衡变化平行。这些结果表明，饮食组成与肾上腺分泌相互作用，影响ob/ob小鼠的能量和葡萄糖代谢；当肾上腺切除ob/ob小鼠食用非纯化饮食时，食用纯化高葡萄糖或高脂饮食都会抵消肾上腺切除术对能量和葡萄糖代谢的有益影响。
