Mercer S W, Trayhurn P
Dunn Nutrition Laboratory, Medical Research Council, Cambridge, United Kingdom.
J Nutr. 1987 Dec;117(12):2147-53. doi: 10.1093/jn/117.12.2147.
The effects on energy balance and brown adipose tissue thermogenesis of feeding high fat diets of differing fatty acid composition have been investigated in lean and genetically obese (ob/ob) mice. Groups of mice were fed either a low fat diet or a high fat diet based on corn oil or beef tallow for 2 wk. Energy intake and body weight gain were higher in both lean and obese animals fed the high fat diets than in respective mice fed the low fat diets. Carcass energy gain was greater for the obese than for the lean consuming each of the diets. Both lean and obese mice had a higher energy gain when fed the beef tallow diet than when fed the corn oil, despite isoenergetic intakes of the two diets. The thermogenic activity of brown adipose tissue, assessed from measurements of cytochrome oxidase activity and mitochondrial guanosine 5'-diphosphate (GDP) binding, were greater in both lean and obese mice fed the corn oil diet than in those fed the low fat diet. However, GDP binding and cytochrome oxidase activities in lean or obese mice fed the beef tallow diet were not different from those of mice of the same genotype fed the low fat diet. These results indicate that in both lean and obese (ob/ob) mice energy deposition and the stimulation of brown adipose tissue thermogenesis during the voluntary hyperphagia induced by feeding high fat diets are influenced by the fatty acid composition of the diet. A diet rich in polyunsaturated fatty acids appears to result in preferential stimulation of the thermogenic activity of brown adipose tissue, particularly in the ob/ob mouse.
在瘦小鼠和遗传性肥胖（ob/ob）小鼠中，研究了不同脂肪酸组成的高脂饮食对能量平衡和棕色脂肪组织产热的影响。将小鼠分组，分别喂食基于玉米油或牛脂的低脂饮食或高脂饮食，持续2周。与喂食低脂饮食的相应小鼠相比，喂食高脂饮食的瘦小鼠和肥胖小鼠的能量摄入量和体重增加都更高。在食用每种饮食时，肥胖小鼠的胴体能量增加比瘦小鼠更大。尽管两种饮食的能量摄入相等，但喂食牛脂饮食的瘦小鼠和肥胖小鼠的能量增加都高于喂食玉米油饮食的小鼠。通过测量细胞色素氧化酶活性和线粒体鸟苷5'-二磷酸（GDP）结合来评估棕色脂肪组织的产热活性，结果发现，喂食玉米油饮食的瘦小鼠和肥胖小鼠的产热活性均高于喂食低脂饮食的小鼠。然而，喂食牛脂饮食的瘦小鼠或肥胖小鼠的GDP结合和细胞色素氧化酶活性与喂食低脂饮食的同基因型小鼠没有差异。这些结果表明，在瘦小鼠和肥胖（ob/ob）小鼠中，高脂饮食诱导的自愿性多食期间的能量沉积和棕色脂肪组织产热的刺激都受饮食中脂肪酸组成的影响。富含多不饱和脂肪酸的饮食似乎会优先刺激棕色脂肪组织的产热活性，尤其是在ob/ob小鼠中。
