School of Engineering, Westlake University, Hangzhou, 310024, China.
Vantronics Hangzhou Intelligence Technology Ltd., Hangzhou, 311100, China.
Nat Commun. 2022 Aug 20;13(1):4902. doi: 10.1038/s41467-022-32676-4.
A lab-on-a-chip system with Point-of-Care testing capability offers rapid and accurate diagnostic potential and is useful in resource-limited settings where biomedical equipment and skilled professionals are not readily available. However, a Point-of-Care testing system that simultaneously possesses all required features of multifunctional dispensing, on-demand release, robust operations, and capability for long-term reagent storage is still a major challenge. Here, we describe a film-lever actuated switch technology that can manipulate liquids in any direction, provide accurate and proportional release response to the applied pneumatic pressure, as well as sustain robustness during abrupt movements and vibrations. Based on the technology, we also describe development of a polymerase chain reaction system that integrates reagent introduction, mixing and reaction functions all in one process, which accomplishes "sample-in-answer-out" performance for all clinical nasal samples from 18 patients with Influenza and 18 individual controls, in good concordance of fluorescence intensity with standard polymerase chain reaction (Pearson coefficients > 0.9). The proposed platform promises robust automation of biomedical analysis, and thus can accelerate the commercialization of a range of Point-of-Care testing devices.
一种具有即时检测能力的片上实验室系统具有快速、准确的诊断潜力，适用于生物医学设备和熟练专业人员不易获得的资源有限的环境。然而，一个同时具备多功能分配、按需释放、稳健操作以及长期试剂储存能力的即时检测系统仍然是一个主要挑战。在这里，我们描述了一种薄膜杠杆驱动开关技术，该技术可以在任何方向上操纵液体，对施加的气压产生精确和比例的释放响应，并在突然的运动和振动中保持稳健性。基于该技术，我们还描述了聚合酶链反应系统的开发，该系统将试剂引入、混合和反应功能集成在一个过程中，对于来自 18 名流感患者和 18 名个体对照者的所有临床鼻样本，都能实现“样本进结果出”的性能，荧光强度与标准聚合酶链反应非常吻合（皮尔逊系数>0.9）。该平台有望实现稳健的生物医学分析自动化，从而加速一系列即时检测设备的商业化。
