Department of Oral Medicine, Peking University School and Hospital of Stomatology, National Center of Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health, NMPA Key Laboratory for Dental Materials, Beijing, P.R. China.
J Prosthodont. 2023 Jul;32(6):505-511. doi: 10.1111/jopr.13595. Epub 2022 Sep 13.
This study aimed to explore the antimicrobial properties of graphene coated Ti-6Al-4V to oral pathogens.
Graphene directly synthesized on Ti-6Al-4V alloy was characterized by scanning electron microscopy (SEM) and Raman spectroscopy. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, live/dead fluorescent staining and SEM were used to analyze the antimicrobial properties of graphene coated Ti-6Al-4V alloy to Porphyromonas gingivalis (P. gingivalis), Fusobacterium nucleatum (F. nucleatum), and Candida albicans (C. albicans). Reactive oxygen species (ROS) generation was monitored to reveal the antimicrobial mechanism.
Graphene coated Ti-6Al-4V alloy caused a significant reduction in the presence of both bacterial and fungal pathogens as compared to uncoated Ti-6Al-4V alloy. P. gingivalis, F. nucleatum, and C. albicans on graphene coated Ti-6Al-4V alloy were less active than on uncoated Ti-6Al-4V alloy, and tended to become shrunk and deformed. Meanwhile, graphene coated Ti-6Al-4V alloy induced more generation of ROS in the pathogens than uncoated Ti-6Al-4V alloy.
Graphene coated Ti-6Al-4V alloy exhibited antimicrobial properties against oral pathogens, the induction of oxidative stress might be involved in its antimicrobial mechanisms.
本研究旨在探索涂覆石墨烯的 Ti-6Al-4V 对口腔病原体的抗菌性能。
采用扫描电子显微镜（SEM）和拉曼光谱对直接在 Ti-6Al-4V 合金上合成的石墨烯进行了表征。采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法、活/死荧光染色和 SEM 分析了涂覆石墨烯的 Ti-6Al-4V 合金对牙龈卟啉单胞菌（P. gingivalis）、核梭杆菌（F. nucleatum）和白色念珠菌（C. albicans）的抗菌性能。监测活性氧（ROS）的产生以揭示其抗菌机制。
与未涂覆的 Ti-6Al-4V 合金相比，涂覆石墨烯的 Ti-6Al-4V 合金显著减少了两种细菌和真菌病原体的存在。涂覆石墨烯的 Ti-6Al-4V 合金上的 P. gingivalis、F. nucleatum 和 C. albicans 比未涂覆的 Ti-6Al-4V 合金上的更不活跃，并且趋于收缩和变形。同时，涂覆石墨烯的 Ti-6Al-4V 合金在病原体中诱导产生的 ROS 比未涂覆的 Ti-6Al-4V 合金更多。
涂覆石墨烯的 Ti-6Al-4V 合金对口腔病原体具有抗菌性能，氧化应激的诱导可能与其抗菌机制有关。
