Institute of Resource Ecology, Department of Biogeochemistry, Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstr. 400, 01328 Dresden, Germany.
Institute of Resource Ecology, Department of Biogeochemistry, Helmholtz-Zentrum Dresden-Rossendorf, Bautzner Landstr. 400, 01328 Dresden, Germany.
Sci Total Environ. 2022 Dec 10;851(Pt 2):158160. doi: 10.1016/j.scitotenv.2022.158160. Epub 2022 Aug 19.
Europium, as an easy-to-study analog of the trivalent actinides, is of particular importance for studying the behavior of lanthanides and actinides in the environment. Since different soil organisms can influence the migration behavior of these elements, a detailed knowledge of these interaction mechanisms is important. The aim of this study was to investigate the interaction of mycelia of selected wood-inhabiting (S. commune, P. ostreatus, L. tigrinus) and soil-inhabiting fungi (L. naucinus) with Eu(III). In addition to determining the Eu(III) complexes in the sorption solution, the formed Eu(III) fungal species were characterized using scanning transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy, chemical microscopy in combination with the time-resolved laser-induced fluorescence spectroscopy. Our data show that S. commune exhibited significantly higher Eu(III) binding capacity in comparison to the other fungi. Depending on fungal strain, the metal was immobilized on the cell surface, in the cell membranes, and within the membranes of various organelles, or in the cytoplasm in some cases. During the bioassociation process two different Eu(III) fungal species were formed in all investigated fungal strain. The phosphate groups of organic ligands were identified as being important functional groups to bind Eu(III) and thus immobilize the metal in the fungal matrix. The information obtained contributes to a better understanding of the role of fungi in migration, removal or retention mechanisms of rare earth elements and trivalent actinides in the environment.
铕作为三价锕系元素的易于研究的类似物，对于研究环境中镧系元素和锕系元素的行为具有特别重要的意义。由于不同的土壤生物可以影响这些元素的迁移行为，因此详细了解这些相互作用机制非常重要。本研究的目的是研究选定的木质素（S. commune、P. ostreatus、L. tigrinus）和土壤真菌（L. naucinus）的菌丝与 Eu(III)的相互作用。除了确定吸附溶液中的 Eu(III)配合物外，还使用扫描透射电子显微镜结合能量色散 X 射线光谱、化学显微镜结合时间分辨激光诱导荧光光谱对形成的 Eu(III)真菌物种进行了表征。我们的数据表明，与其他真菌相比，S. commune 表现出更高的 Eu(III)结合能力。根据真菌菌株的不同，金属被固定在细胞表面、细胞膜内和各种细胞器的膜内，或者在某些情况下固定在细胞质中。在生物结合过程中，在所研究的所有真菌菌株中都形成了两种不同的 Eu(III)真菌物种。有机配体的磷酸基团被确定为结合 Eu(III)并将金属固定在真菌基质中的重要功能基团。所获得的信息有助于更好地了解真菌在环境中迁移、去除或保留稀土元素和三价锕系元素的机制中的作用。
