Qi Yi, Ma Pei, Wang Pan, Zhu Chenhui
Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry·of Education, School of Chemical Engineering, Northwest University, Xi'an 710127, China.
Shaanxi Key Laboratory of Biomaterials and Synthetic Biology, Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, Xi'an 710127, China.
Foods. 2025 Jun 10;14(12):2049. doi: 10.3390/foods14122049.
Ginseng peptides, as bioactive components of ginseng, have attracted increasing attention. In this study, a 14-amino acid ginseng peptide was selected and heterologously expressed in using a multicopy tandem fusion strategy, named 7RS14α. The secondary structure of the recombinant ginseng tetradecapeptide (7RS14α) was analyzed, and a high-glucose model was established in mouse adipocytes to evaluate its biological activity. Transcriptomic profiling was further performed to elucidate its potential mechanisms. Results demonstrated that 7RS14α significantly enhanced glucose uptake in high-glucose model cells, likely by modulating lipid metabolism pathways and insulin signaling cascades, thereby influencing energy homeostasis in adipocytes.
人参肽作为人参的生物活性成分，已引起越来越多的关注。在本研究中，选择了一种14个氨基酸的人参肽，并采用多拷贝串联融合策略在[具体表达系统未给出]中进行异源表达，命名为7RS14α。分析了重组人参十四肽（7RS14α）的二级结构，并在小鼠脂肪细胞中建立高糖模型以评估其生物活性。进一步进行转录组分析以阐明其潜在机制。结果表明，7RS14α显著增强了高糖模型细胞中的葡萄糖摄取，可能是通过调节脂质代谢途径和胰岛素信号级联反应，从而影响脂肪细胞中的能量稳态。
