Ganji Ali, Jalali-Mashayekhi Farideh, Hajihossein Reza, Eslamirad Zahra, Bayat Parvin-Dokht, Sakhaie Mohammadhasan
Department of Microbiology and Immunology, School of Medicine, Arak University of Medical Sciences, Arak, Iran; Molecular and Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Exp Parasitol. 2022 Oct;241:108360. doi: 10.1016/j.exppara.2022.108360. Epub 2022 Aug 19.
The main goal of the current study was to evaluate the effectiveness of resveratrol (RESV) on protoscolices and hydatid cysts of Echinococcus granolosus. Echinococcus granolosus protoscolices and hydatid cyst were exposed to RPMI, DMSO, formalin, mebendazole, and different concentrations of RESV in vitro. Then, viability, GGT, and caspase-3 activity of protoscolices were evaluated using light microscopy, colorimetric, and enzymatic assay, respectively. Tissue changes and expression of caspase-3 apoptosis were analyzed on the hydatid cyst wall by histologic and immunohistochemistry methods. The cell toxicity effect of RESV was evaluated on mouse PBMCs by Annexin V-FITC assay. The RESV-treated protoscolices showed loss of viability, increased gamma-glutamyl transpeptidase, and caspase-3 activity with significant differences compared to all control groups (P < 0.05). Dose and time dependence of mortality, GGT, and caspase-3 enzymatic activity was confirmed in the protoscolices of Echinococcus granulosus treated by RESV. Also, the tissue changes and apoptosis were prominent in RESV-treated hydatid cyst layers; however, tissue changes were only time-dependent, and RESV concentration had no apparent effect on tissue. In cell toxicity evaluation, RESV is safe without any significant apoptosis induction from 31.5 to 250 μg/ml; however, it was significant at 350 and 500 μg/ml in PBMCs.
本研究的主要目的是评估白藜芦醇（RESV）对细粒棘球绦虫原头节和包虫囊肿的有效性。将细粒棘球绦虫原头节和包虫囊肿在体外分别暴露于RPMI、二甲基亚砜、福尔马林、甲苯达唑和不同浓度的RESV中。然后，分别使用光学显微镜、比色法和酶法测定原头节的活力、γ-谷氨酰转肽酶（GGT）和半胱天冬酶-3活性。通过组织学和免疫组织化学方法分析包虫囊肿壁上的组织变化和半胱天冬酶-3凋亡的表达。通过膜联蛋白V-异硫氰酸荧光素（Annexin V-FITC）测定法评估RESV对小鼠外周血单核细胞（PBMC）的细胞毒性作用。与所有对照组相比，经RESV处理的原头节显示活力丧失、γ-谷氨酰转肽酶增加和半胱天冬酶-3活性增加，差异有统计学意义（P<0.05）。RESV处理的细粒棘球绦虫原头节中，死亡率、GGT和半胱天冬酶-3酶活性的剂量和时间依赖性得到证实。此外，RESV处理的包虫囊肿层中组织变化和凋亡明显；然而，组织变化仅与时间有关，RESV浓度对组织无明显影响。在细胞毒性评估中，RESV在31.5至250μg/ml时是安全的，没有任何明显的凋亡诱导作用；然而，在PBMC中，350和500μg/ml时具有显著作用。
