Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Basic Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Int J Nanomedicine. 2020 Feb 17;15:1095-1100. doi: 10.2147/IJN.S228538. eCollection 2020.
is causative agent of cystic echinococcosis (CE), which has a cosmopolitan distribution. The current methods for the treatment of human CE include surgery. Therefore, the development of new scolicidal agents with low side effects and more efficacies is an urgent need.
The present study aimed to compare the scolicidal efficacies of silver, iron, copper, silica and zinc oxide nanoparticles (NPs) against hydatid cyst protoscolices in vitro.
Hydatid cysts of sheep liver and lung were collected. The cyst fluid containing protoscolices was aspirated aseptically. The scolicidal activities of the silver, iron, copper, silica and zinc nanoparticles (Ag-NP, Fe-NP, Cu-NP, Si-NP and Zn-NP) were tested at different concentrations of 0.25, 0.5 and 1 mg/mL following 10, 30 and 60 min of incubation in triplicate. Viability of protoscolices was confirmed by 0.1% eosin staining.
Results showed that Ag-NPs at all concentrations tested had the highest scolicidal effect. Ag-NPs at 1 mg/mL concentration after 60 min of exposure time showed 80% mortality rate. Si-NPs had the high scolicidal activity at 1 mg/mL concentration (52.33%), Cu-NPs at 0.5 mg/mL concentration (41%), Fe-NPs at 1mg/mL concentration (28%) and Zn-NPs at concentration of 1mg/mL after 60 mins (15.67%).
The findings of the present study showed that Ag-NPs, Fe-NPs, Cu-NPs, Si-NPs and Zn-NPs had potent scolicidal effects and that Ag-NPs are recommended as effective scolicidal agents. However, further in vivo studies are required to evaluate the efficacy of these nanoparticles.
是囊型包虫病（CE）的病原体，分布广泛。目前治疗人类 CE 的方法包括手术。因此，开发副作用低、疗效更高的新杀囊剂是当务之急。
本研究旨在比较银、铁、铜、硅和氧化锌纳米粒子（NP）对体外包虫蚴原头节的杀囊效果。
采集绵羊肝、肺包虫囊肿。无菌抽吸含原头节的囊液。分别用浓度为 0.25、0.5 和 1 mg/mL 的银、铁、铜、硅和氧化锌纳米粒子（Ag-NP、Fe-NP、Cu-NP、Si-NP 和 Zn-NP）在 10、30 和 60 分钟孵育后，重复 3 次测试杀囊活性。用 0.1%曙红染色确认原头节的活力。
结果表明，所有测试浓度的 Ag-NP 均具有最高的杀囊效果。暴露 60 分钟后，浓度为 1mg/mL 的 Ag-NP 表现出 80%的死亡率。1mg/mL 浓度的 Si-NP 具有最高的杀囊活性（52.33%），0.5mg/mL 浓度的 Cu-NP（41%），1mg/mL 浓度的 Fe-NP（28%）和 60 分钟后浓度为 1mg/mL 的 Zn-NP（15.67%）。
本研究结果表明，Ag-NP、Fe-NP、Cu-NP、Si-NP 和 Zn-NP 具有较强的杀囊作用，Ag-NP 被推荐为有效的杀囊剂。然而，需要进一步的体内研究来评估这些纳米粒子的疗效。
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