Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.
Department of Marine Biology, Faculty of Marine Science and Technology, University of Hormozgan, Bandar Abbas, Iran.
Exp Parasitol. 2019 Aug;203:19-22. doi: 10.1016/j.exppara.2019.05.009. Epub 2019 May 30.
Hydatid cyst is a zoonotic parasitic infection caused by the tapeworm Echinococcus granulosus. Such infections are of considerable public health and economic concern, and new effective treatments are intensely sought. Sea urchin (Salmacis virgulata) shell extracts have potent antimicrobial and antioxidant activity, and spines of several species of echinoderms also show antimicrobial activity. In the present in vitro study, we investigated the scolicidal effect of spines and shells extractions from Echinometra mathaei obtained from the Persian Gulf. Spines and shells from the sea urchin, Echinometra mathaei were used in the tests. Spines and shells from 800 specimens were extracted with dibasic sodium phosphate buffer (pH 7.5). Procedures used protoscolices of E. granulosus were obtained aseptically from hydatid cyst in naturally infected sheep's liver and goats and viable protoscolices exposed with spine and shell extractions. The apoptosis was assessed by measuring the caspase 3 activity of the extract-treated protoscolices, using ELISA-based commercial kits to determine caspase activity. The scolicidal effects of shells were also showed, 20 μg/ml of shell extracts after 60 min exposure, the viability of protoscolices were 21.99 ± 0.01. The results showed that 20 μg/ml of spines gave maximum scolicidal activity (p < 0.05). This study represents the first attempt at combatting echinoid parasites by natural compounds with high efficiency, and may provide a base for future treatment of hydatid cysts.
棘球蚴病是一种由带绦虫细粒棘球绦虫引起的人畜共患寄生虫感染。这种感染对公共卫生和经济有相当大的影响，因此人们强烈寻求新的有效治疗方法。海胆（Salmacis virgulata）壳提取物具有很强的抗菌和抗氧化活性，几种棘皮动物的刺也具有抗菌活性。在本体外研究中，我们研究了从波斯湾获得的海胆（Echinometra mathaei）的棘和壳提取物的杀囊作用。 棘和壳来自棘皮动物海胆 Echinometra mathaei。 使用磷酸二氢钠缓冲液（pH 7.5）从 800 个标本中提取棘和壳。 从自然感染绵羊和山羊肝脏的包虫囊肿中无菌获得细粒棘球蚴原头蚴，并将其暴露于棘和壳提取物中。 通过 ELISA 试剂盒测定提取物处理的原头蚴中半胱天冬酶 3 活性来评估细胞凋亡。 暴露 60 分钟后，壳提取物的浓度为 20 μg/ml，原头蚴的存活率为 21.99±0.01。 结果表明，20 μg/ml 的棘具有最大的杀囊作用（p<0.05）。 这项研究代表了首次尝试使用高效的天然化合物来对抗海胆寄生虫，为未来治疗包虫囊肿提供了基础。
