State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, Xinjiang, 830011, PR China.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
Int J Syst Evol Microbiol. 2022 Aug;72(8). doi: 10.1099/ijsem.0.005503.
A novel actinobacterial strain, designated SYSU K20354, was isolated from a soil sample collected from a karst cave in Shaoguan city, Guangdong province, southern China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic, Gram-stain-positive and non-motile. On the basis of 16S rRNA gene sequence similarities and phylogenetic analysis, strain SYSU K20354 was most closely related to JCM 14319, and shared the highest sequence identity of 98.3 % based on NCBI database. In addition, 2,4-diaminobutyric acid was the diagnostic diamino acid in cell-wall peptidoglycan. The whole-cell sugars were galactose, glucose, mannose and ribose. The major isoprenoid quinone was MK-12, while the major fatty acids (>10 %) were isoC, anteisoC and anteisoC. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, three unknown glycolipids, three unknown phospholipids and two unknown lipids. The draft genome size of strain SYSU K20354 was 3.96 Mbp with G+C content of 69.7 mol%. Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain SYSU K20354 and JCM 14319 were 90.3 and 55.6 %, respectively. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K20354 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU K20354 (=KCTC 49499= CGMCC 4.7691).
一株新的放线菌菌株，命名为 SYSU K20354，从中国南部广东省韶关市一个喀斯特洞穴的土壤样本中分离得到。该菌株的分类地位通过多相研究方法进行了研究。该菌株细胞为需氧、革兰氏阳性、非运动性。根据 16S rRNA 基因序列相似性和系统发育分析，菌株 SYSU K20354与 JCM 14319 最为密切相关，基于 NCBI 数据库，其序列相似度最高为 98.3%。此外，细胞壁肽聚糖中的特征二氨基庚二酸是 2,4-二氨基丁酸。全细胞糖为半乳糖、葡萄糖、甘露糖和核糖。主要的异戊烯醌为 MK-12，而主要的脂肪酸（>10%）为 isoC、anteisoC 和 anteisoC。极性脂含有双磷脂酰甘油、磷脂酰甘油、三种未知糖脂、三种未知磷脂和两种未知脂。菌株 SYSU K20354 的基因组草图大小为 3.96 Mbp，GC 含量为 69.7 mol%。此外，菌株 SYSU K20354 与 JCM 14319 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 90.3%和 55.6%。基于表型、基因型和系统发育数据，菌株 SYSU K20354 代表了属的一个新种，提议使用新种名 sp. nov.。模式菌株为 SYSU K20354（=KCTC 49499=CGMCC 4.7691）。
Zotero 插件