Department of Biological Science, College of Science and Engineering, Sangji University, Wonju 26339, Republic of Korea.
Department of Microbiology, Pukyong National University, Busan 48513, Republic of Korea.
J Microbiol Biotechnol. 2024 Jul 28;34(7):1475-1483. doi: 10.4014/jmb.2404.04007. Epub 2024 May 23.
Three Gram-stain-positive, aerobic, rod-shaped, and non-motile bacteria, labelled as W11, SW19, and YR1, were isolated from soil, and performed their polyphasic taxonomic investigation. The phylogenetic and 16S rRNA gene sequence analysis showed that strains W11, SW19, and YR1 belonged to the genera , , and , respectively. Strain W11 was closely affiliated with SYSU K20354 (98.1%), strain SW19 showed the closest affiliation with ZW T2_19 (97.0%), and strain YR1 was most closely related to KIS18-7 (98.0%). The genome sizes of strains W11, SW19, and YR1 were 4,181,720 bp, 4,740,677 bp, and 4,228,226 bp, respectively, with DNA G+C contents of 70.5%, 64.2%, and 69.7%, respectively. Average nucleotide identity and digital DNA-DNA hybridization values of W11, SW19, and YR1 with their respective reference species were <79.6% and <23.6%, respectively. The predominant cellular fatty acids detected in strain W11 were anteiso-C, iso-C, and anteiso-C. In strain SW19, they were summed feature 9 (C 10-methyl and/or iso-Cω 9c), anteiso-C, and anteiso-C. Strain YR1 exhibited Cω 9c, C 10-methyl, TBSA, and anteiso-C as its major cellular fatty acids. Overall, the polyphasic taxonomic comparisons indicated that strains W11, SW19, and YR1 represent novel species within the genera , , and , respectively. Accordingly, we propose the names sp. nov., with the type strain W11 (=KCTC 49818 =NBRC 115999), sp. nov., with the type strain SW19 (=KCTC 49860 =NBRC 116108), and sp. nov., with the type strain YR1 (=KCTC 49863 =NBRC 116165).
从土壤中分离到三株革兰氏阳性、需氧、杆状且不运动的细菌，分别命名为 W11、SW19 和 YR1，并对其进行了多相分类学研究。基于系统发育和 16S rRNA 基因序列分析，菌株 W11、SW19 和 YR1 分别属于 、 和 属。菌株 W11 与 SYSU K20354（98.1%）密切相关，SW19 与 ZW T2_19（97.0%）最相似，而 YR1 与 KIS18-7（98.0%）最为相似。菌株 W11、SW19 和 YR1 的基因组大小分别为 4181720bp、4740677bp 和 4228226bp，G+C 含量分别为 70.5%、64.2%和 69.7%。W11、SW19 和 YR1 与各自参考种的平均核苷酸同一性和数字 DNA-DNA 杂交值均<79.6%和<23.6%。菌株 W11 的主要细胞脂肪酸为 anteiso-C、iso-C 和 anteiso-C；SW19 为 summed feature 9（C 10-methyl 和/或 iso-Cω 9c）、anteiso-C 和 anteiso-C；YR1 为 Cω 9c、C 10-methyl、TBSA 和 anteiso-C。综上，多相分类学比较表明，菌株 W11、SW19 和 YR1 分别代表 、 和 属中的新种。因此，我们建议将菌株 W11 命名为 sp. nov.，其模式菌株为 W11（=KCTC 49818=NBRC 115999）；将菌株 SW19 命名为 sp. nov.，其模式菌株为 SW19（=KCTC 49860=NBRC 116108）；将菌株 YR1 命名为 sp. nov.，其模式菌株为 YR1（=KCTC 49863=NBRC 116165）。
