Van Vliet F, Couturier M, De Lafonteyne J, Jedlicki E
Mol Gen Genet. 1978 Aug 4;164(1):109-12. doi: 10.1007/BF00267606.
The spontaneous DNA breakdown exhibited by recA strains, is reduced after heat induction of a thermoinducible Mu-1 prophage. This inhibition is dependent upon RNA synthesis, suggesting that Mu-1 directs synthesis of a recBC nuclease inhibitor, analogous to the product of the lambda gam gene. The genetic evidence presented here shows that Mu-1 enables a lambda red gam phage to grow on a recA host. The in vitro assay for ATP-dependent exonuclease activity reveals a complete inhibition of this activity 30 min after induction of the Mu-1 prophage.
recA菌株所表现出的自发DNA降解，在热诱导可热诱导的Mu-1原噬菌体后会减少。这种抑制作用依赖于RNA合成，这表明Mu-1指导合成一种recBC核酸酶抑制剂，类似于λgam基因的产物。此处提供的遗传学证据表明，Mu-1能使λred gam噬菌体在recA宿主上生长。对ATP依赖性核酸外切酶活性的体外测定显示，在诱导Mu-1原噬菌体30分钟后，这种活性被完全抑制。
