Department of Chemistry "Giacomo Ciamician", Alma Mater Studiorum, University of Bologna, Via Francesco Selmi 2, I-40126 Bologna, Italy.
Interdepartmental Centre for Industrial Aerospace Research (CIRI AEROSPACE), Alma Mater Studiorum, University of Bologna, Via Baldassarre Canaccini 12, I-47121 Forlì, Italy.
Biosensors (Basel). 2022 Aug 15;12(8):644. doi: 10.3390/bios12080644.
The presence of hidden allergens in food products, often due to unintended contamination along the food supply chain (production, transformation, processing, and transport), has raised the urgent need for rapid and reliable analytical methods for detecting trace levels of such species in food products. Indeed, food allergens represent a high-risk factor for allergic subjects due to potentially life-threatening adverse reactions. Portable biosensors based on immunoassays have already been developed as rapid, sensitive, selective, and low-cost analytical platforms that can replace analyses with traditional bench-top instrumentation. Recently, aptamers have attracted great interest as alternative biorecognition molecules for bioassays, since they can bind a variety of targets with high specificity and selectivity, and they enable the development of assays exploiting a variety of transduction and detection technologies. In particular, aptasensors based on luminescence detection have been proposed, taking advantage of the development of ultrasensitive tracers and enhancers. This review aims to summarize and discuss recent efforts in the field of food allergen analysis using aptamer-based bioassays with luminescence detection.
食品中隐藏过敏原的存在，通常是由于食品供应链（生产、转化、加工和运输）中的意外污染，这就迫切需要快速可靠的分析方法来检测食品中痕量此类物质。事实上，由于潜在的危及生命的不良反应，食物过敏原对过敏体质者来说是一个高风险因素。基于免疫测定的便携式生物传感器已经被开发为快速、灵敏、选择性和低成本的分析平台，可以替代传统台式仪器的分析。最近，适配体作为生物分析的替代生物识别分子引起了极大的兴趣，因为它们可以与多种靶标结合，具有很高的特异性和选择性，并能够开发利用多种转导和检测技术的分析方法。特别是，已经提出了基于发光检测的适配体传感器，利用超灵敏示踪剂和增强剂的发展。本综述旨在总结和讨论利用基于适配体的生物发光检测法进行食品过敏原分析的最新进展。
