Xu Runfeng, Chen Jie, Pan Yu, Wang Jiachen, Chen Lu, Ruan Honghua, Wu Yongbo, Xu Hanmei, Wang Guobing, Liu Hongyi
The Co-Innovation Center for Sustainable Forestry in Southern China, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
Key Laboratory for Ecology and Pollution Control of Coastal Wetlands (Environmental Protection, Department of Jiangsu), School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224007, China.
Insects. 2022 Aug 15;13(8):729. doi: 10.3390/insects13080729.
Soil macrofauna, such as , are an important component of ecosystems. However, systematic studies of the genetic diversity, population genetic structure, and the potential factors affecting the genetic differentiation of are lacking. We performed a population genetic study of 166 individuals from the mountains to the south of the Yangtze River, north of the Yangtze River in Nanjing city, and near Tianjin city, in order to investigate the correlations between geographical distance and genetic diversity. A total of 1182 bp of and gene sequences of mitochondrial DNA, and 700 bp of the 18S rRNA gene sequence were analyzed. There were two haplotypes and one variable site in the 18S rRNA gene, and 28 haplotypes and 78 variable sites in the and genes. In this study, the 18S rRNA gene was used for species identification, and mtDNA (concatenated sequences with and ) was used for population genetic analysis. Structure cluster analysis indicated that the genetic structures of the different populations of tended to be consistent at small geographical scales. Phylogenetic trees revealed that the haplotypes were clearly divided into three branches: the area south of the Yangtze River, the area to the north of the Yangtze River in Nanjing, and the area in Tianjin. Large geographical barriers and long geographical distance significantly blocked gene flow between populations of . Our results provide a basic theoretical basis for subsequent studies of millipede taxonomy and population genetic evolution.
土壤大型动物，如 ，是生态系统的重要组成部分。然而，目前缺乏对其遗传多样性、种群遗传结构以及影响其遗传分化的潜在因素的系统研究。我们对长江以南山区、南京市长江以北地区以及天津市附近的166个个体进行了种群遗传学研究，以探究地理距离与遗传多样性之间的相关性。共分析了线粒体DNA的 和 基因序列的1182 bp，以及18S rRNA基因序列的700 bp。18S rRNA基因中有两个单倍型和一个可变位点， 和 基因中有28个单倍型和78个可变位点。在本研究中，18S rRNA基因用于物种鉴定，线粒体DNA（ 和 的串联序列）用于种群遗传分析。结构聚类分析表明，在较小的地理尺度上，不同种群的 遗传结构趋于一致。系统发育树显示，单倍型明显分为三个分支：长江以南地区、南京市长江以北地区和天津市地区。较大的地理障碍和较长的地理距离显著阻碍了 种群之间的基因流动。我们的研究结果为后续马陆分类学和种群遗传进化研究提供了基础理论依据。
