College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, People's Republic of China.
Int J Biol Macromol. 2021 Jul 31;183:1079-1086. doi: 10.1016/j.ijbiomac.2021.05.049. Epub 2021 May 10.
Generally, a teleostean group possesses only one type or a set of similar mitochondrial gene arrangements. However, a new type of gene arrangement has been identified in the mitochondrial genomes (mitogenomes) of Moenkhausia. Here, three newly sequenced complete mitogenomes of tetras (Characidae: Moenkhausia) are presented (M. costae, M. pittieri, and M. sanctaefilomenae). The three mitogenomes had a classical circular structure, with total lengths ranging from 15,811 to 18,435 bp. Base composition analysis indicated that the sequences were biased toward adenine (A) and thymine (T), with A + T content of 54.63% in M. costae, 58.47% in M. pittieri, and 59.98% in M. sanctaefilomenae. The gene order and organization of M. sanctaefilomenae differed from those of typical teleostean mitogenomes. The genes tRNA-Ile, tRNA-Gln, and tRNA-Pro were translocated between tRNA-Trp and tRNA-Asn. One extra tRNA-Met and an extra CR were also discovered in the mitogenome. BI and ML analyses based on sequences of 38 different mitogenomes showed that M. costae and M. pittieri were classified together, and M. sanctaefilomenae was slightly further from other fish of the same genus. These results provide insight into the gene arrangement features of Characidae mitogenomes and lay the foundation for further phylogenetic studies on Characidae.
一般来说，硬骨鱼类的线粒体基因组（mitogenome）仅具有一种或一组相似的基因排列。然而，在 Moenkhausia 的线粒体基因组中发现了一种新的基因排列类型。这里呈现了三种新测序的四齿鱼科（Characidae：Moenkhausia）完整线粒体基因组（M. costae、M. pittieri 和 M. sanctaefilomenae）。这三个线粒体基因组具有经典的圆形结构，总长范围为 15811 到 18435bp。碱基组成分析表明，序列偏向腺嘌呤（A）和胸腺嘧啶（T），A+T 含量在 M. costae 中为 54.63%，在 M. pittieri 中为 58.47%，在 M. sanctaefilomenae 中为 59.98%。M. sanctaefilomenae 的基因顺序和组织与典型的硬骨鱼类线粒体基因组不同。tRNA-Ile、tRNA-Gln 和 tRNA-Pro 基因在 tRNA-Trp 和 tRNA-Asn 之间发生了易位。在线粒体基因组中还发现了一个额外的 tRNA-Met 和一个额外的 CR。基于 38 个不同线粒体基因组序列的 BI 和 ML 分析表明，M. costae 和 M. pittieri 被归为一类，而 M. sanctaefilomenae 与同属的其他鱼类略有不同。这些结果为 Characidae 线粒体基因组的基因排列特征提供了深入的了解，并为进一步的 Characidae 系统发育研究奠定了基础。
