Division of Genetics and Plant Breeding, Faculty of Agriculture (FoA), SKUAST-Kashmir, Wadura Campus, Sopore, Kashmir, India.
Department of Botany, Cytogenetics and Reproductive Biology Laboratory, University of Kashmir, Srinagar, Kashmir, India.
Mol Biol Rep. 2022 Dec;49(12):11469-11479. doi: 10.1007/s11033-022-07858-4. Epub 2022 Aug 25.
The exploration of genetic diversity is the key source of germplasm conservation and potential to broaden its genetic base. The globally growing demand for chickpea suggests superior/climate-resilient varieties, which in turn necessitates the germplasm characterization to unravel underlying genetic variation.
A chickpea core collection comprising of diverse 192 accessions which include cultivated Cicer arietinum, and wild C. reticulatum, C. echinospermum, and C. microphyllum species were investigated to analyze their genetic diversity and relationship, by assaying 33 unlinked simple sequence repeat (SSR) markers. The results amplified a total of 323 alleles (Na), ranging from 2 to 8 with an average of 4.25 alleles per locus. Expected heterozygosity (He) differed from 0.46 to 0.86 with an average of 0.68. Polymorphic information content (PIC) ranged from 0.73 to 0.98 with an average of 0.89. Analysis of molecular variance (AMOVA) showed that most of the variation was among individuals (87%). Cluster analysis resulted in the formation of four distinct clusters. Cluster I represented all cultivated and clusters II, III, and IV comprised a heterogeneous group of cultivated and wild chickpea accessions.
We report considerable diversity and greater resolving power of SSR markers for assessing variability and interrelationship among the chickpea accessions. The chickpea core is expected to be an efficient resource for breeders for broadening the chickpea genetic base and could be useful for selective breeding of desirable traits and in the identification of target genes for genomics-assisted breeding.
遗传多样性的探索是种质保存和扩大其遗传基础的潜力的关键来源。全球对鹰嘴豆的需求不断增长，这表明需要更好的/抗气候品种，而这反过来又需要对种质进行特征分析，以揭示潜在的遗传变异。
对包括栽培的鹰嘴豆（Cicer arietinum）和野生的 C. reticulatum、C. echinospermum 和 C. microphyllum 物种在内的 192 份多样化的鹰嘴豆核心收集物进行了分析，以研究其遗传多样性和关系，方法是检测 33 个不相关的简单序列重复（SSR）标记。结果共扩增了 323 个等位基因（Na），范围从 2 到 8，平均每个位点有 4.25 个等位基因。期望杂合度（He）从 0.46 到 0.86，平均为 0.68。多态信息含量（PIC）从 0.73 到 0.98，平均为 0.89。分子方差分析（AMOVA）表明，大部分变异是个体之间的（87%）。聚类分析导致形成四个不同的聚类。聚类 I 代表所有的栽培品种，聚类 II、III 和 IV 由栽培和野生鹰嘴豆品种的异质群体组成。
我们报告了相当大的多样性和 SSR 标记更高的分辨率，用于评估鹰嘴豆品种间的变异性和相互关系。鹰嘴豆核心预计将成为培育者扩大鹰嘴豆遗传基础的有效资源，并且可以用于选择理想性状的选择性育种，以及鉴定基因组辅助育种的目标基因。
