Lin M F, Wu C L, Wang T C
Mutat Res. 1987 Jul;188(3):241-50. doi: 10.1016/0165-1218(87)90095-4.
Paraquat, alachlor, butachlor, phorate and monocrotophos, several of the most extensively used pesticides in Taiwan, were investigated for their clastogenicity using chromosome aberration (CAb) induction in Chinese hamster ovary (CHO) cells. Significance levels of the binomial trend analysis and binomial mutagenicity data test were two criteria for the summary judgement of the pesticide clastogenicity. Except for phorate, all pesticides tested were clastogenic to CHO cells in the absence of in vitro metabolic activation by S9. 5 microliters/ml rat-liver extract, S9, were used as the source of in vitro metabolic activation. 3 different outcomes were found after the addition of S9. Paraquat: significant decrease in induced CAbs. Monocrotophos: concomitant occurrence of decreased cytotoxicity and increased clastogenicity. Alachlor, butachlor and phorate: increased cytotoxicities with no sign of enhancement in clastogenicity.
对台湾地区使用最为广泛的几种农药——百草枯、甲草胺、丁草胺、甲拌磷和久效磷，利用中国仓鼠卵巢（CHO）细胞中的染色体畸变（CAb）诱导来研究它们的致断裂性。二项式趋势分析的显著性水平和二项式致突变性数据检验是农药致断裂性总结判断的两个标准。除甲拌磷外，所有测试农药在无S9体外代谢活化的情况下对CHO细胞都有致断裂性。5微升/毫升大鼠肝提取物S9被用作体外代谢活化源。添加S9后发现了3种不同的结果。百草枯：诱导的染色体畸变显著减少。久效磷：细胞毒性降低和致断裂性增加同时出现。甲草胺、丁草胺和甲拌磷：细胞毒性增加，但没有致断裂性增强的迹象。
