School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, 2055 Yanan Street, Changchun, Jilin 130012, P. R. China.
State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin 130022, P. R. China.
ACS Appl Mater Interfaces. 2022 Sep 7;14(35):39858-39865. doi: 10.1021/acsami.2c11596. Epub 2022 Aug 25.
As a representative strategy for cancer immunotherapy, cancer nanovaccines have aroused enormous interest. Although various nanovaccines have been developed to promote immunogenicity and improve the therapeutic efficacy, chiral nanovaccines have been less explored as of yet. Chiral carbon dots (CDs) have similar size to proteins, abundant functional groups, and nanoscale chirality, which can not only carry and deliver antigens but also induce cellular and humoral immune responses and can play dual roles of nanovehicles and immune adjuvants. Herein, we demonstrate that the chiral nanovaccines (l/d-OVA) could be conveniently fabricated by utilizing chiral CDs as carriers and immune adjuvants and ovalbumin (OVA) as an antigen model. l/d-OVA nanovaccines could be effectively internalized by mouse bone-marrow-derived dendritic cells (BMDCs), boost BMDC maturation, efficiently cross-present to T cells, and suppress the growth of B16-OVA melanoma. This work illustrates the hopeful potential of chiral CDs as effective vectors for loading protein cargos and delivering them into cancer cells.
作为癌症免疫疗法的一种代表性策略，癌症纳米疫苗引起了极大的兴趣。尽管已经开发出了各种纳米疫苗来促进免疫原性和提高治疗效果，但手性纳米疫苗的研究还较少。手性碳点（CDs）具有与蛋白质相似的大小、丰富的官能团和纳米级手性，不仅可以携带和递呈抗原，还可以诱导细胞和体液免疫反应，并可以发挥纳米载体和免疫佐剂的双重作用。在这里，我们证明了手性纳米疫苗（l/d-OVA）可以通过利用手性 CD 作为载体和免疫佐剂以及卵清蛋白（OVA）作为抗原模型来方便地制备。l/d-OVA 纳米疫苗可以被小鼠骨髓来源的树突状细胞（BMDCs）有效内化，促进 BMDC 成熟，有效地交叉呈递给 T 细胞，并抑制 B16-OVA 黑色素瘤的生长。这项工作说明了手性 CDs 作为有效载体用于装载蛋白 cargo 并将其递送到癌细胞中的有希望的潜力。
