School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, 2055 Yanan Street, Changchun, Jilin, 130012, P. R. China.
State Key Laboratory of Polymer Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun, Jilin, 130022, P. R. China.
Small. 2023 Aug;19(31):e2206683. doi: 10.1002/smll.202206683. Epub 2023 Mar 28.
Cancer immunotherapy has become one of the current research hotspots. However, the deficiencies including restricted immunogenicity, insufficient antigen presentation, and low responsive rate limited their therapeutic applications. Own to the small size and excellent biocompatibility, carbon dots (CDs) can serve as nanovectors to improve the efficacy of cancer immunotherapy. Herein, a tumor antigen-based nanovaccines (GMal+B16F10-Ag and GMal+CT26-Ag) by the conjugation of CDs with the tumor cell-derived antigens (B16F10-Ag and CT26-Ag) is constructed. These nanovaccines can be effectively taken up by dendritic cells (DC2.4), promote DC cell maturation, cross-present the antigen to T cells, specifically target B16F10 melanoma or CT26 colon cancers, and inhibit tumor growth distinctly. This work illustrates the promise of CDs acting as versatile carriers for antigen delivery to achieve the optimal immunotherapeutic outcomes.
癌症免疫疗法已成为当前的研究热点之一。然而，其免疫原性受限、抗原呈递不足和响应率低等缺陷限制了它们的治疗应用。由于尺寸小、生物相容性好，碳点 (CDs) 可用作纳米载体来提高癌症免疫疗法的疗效。在此，通过将 CDs 与肿瘤细胞衍生抗原（B16F10-Ag 和 CT26-Ag）偶联，构建了基于肿瘤抗原的纳米疫苗（GMal+B16F10-Ag 和 GMal+CT26-Ag）。这些纳米疫苗可以被树突状细胞 (DC2.4) 有效摄取，促进 DC 细胞成熟，将抗原交叉呈递给 T 细胞，特异性靶向 B16F10 黑色素瘤或 CT26 结肠癌，并显著抑制肿瘤生长。这项工作说明了 CDs 作为抗原递呈的多功能载体的潜力，可实现最佳的免疫治疗效果。
