Ghosal D, Saedler H
Nature. 1978 Oct 19;275(5681):611-7. doi: 10.1038/275611a0.
In polar IS2 abolishes galactose operon expression. Operon activity is restored by a 108 base pair mini-insertion within IS2 called IS2--6. The DNA sequences of the gal operon-IS2 junction, the parental IS2 region undergoing sequence rearrangements and IS2--6 itself are reported. IS2--6 is composed of sequence intervals present in both strands of IS2.
在极性条件下,IS2会消除半乳糖操纵子的表达。通过在IS2内一个名为IS2--6的108个碱基对的小插入片段可恢复操纵子活性。本文报道了半乳糖操纵子与IS2连接区、发生序列重排的亲本IS2区域以及IS2--6本身的DNA序列。IS2--6由IS2两条链中都存在的序列间隔组成。