Fusco A, Grieco M, Santoro M, Berlingieri M T, Pilotti S, Pierotti M A, Della Porta G, Vecchio G
Nature. 1987;328(6126):170-2. doi: 10.1038/328170a0.
Using DNA transfection analysis on NIH 3T3 cells, activated human oncogenes have been isolated from a variety of fresh solid tumours. Thyroid neoplasias show a wide range of lesions varying from slowly progressive well-differentiated tumours to anaplastic highly malignant neoplasms. Therefore they represent an attractive model to investigate the role of oncogene activation in different stages of the neoplastic state. Here we report the detection of transforming activity in DNAs extracted from five thyroid papillary carcinomas and two of their respective lymph-nodal metastases.
通过对NIH 3T3细胞进行DNA转染分析，已从多种新鲜实体瘤中分离出活化的人类癌基因。甲状腺肿瘤表现出广泛的病变，从缓慢进展的高分化肿瘤到间变性高恶性肿瘤不等。因此，它们是研究癌基因激活在肿瘤状态不同阶段作用的一个有吸引力的模型。在此，我们报告了从五例甲状腺乳头状癌及其各自的两个淋巴结转移灶中提取的DNA中转化活性的检测结果。
