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[低尿酸人群肠道尿酸降解菌的筛选、驯化及鉴定]

[Screening, domestication and identification of intestinal uric acid degrading bacteria in low uric acid population].

作者信息

Tian Tingting, Chen Wujin, Liang Meiting, Li Rui, Sun Yuping

机构信息

Basic Medical College, Xinjiang Medical University, Urumqi 830000, P. R. China.

Morphological Center, Basic Medical College, Xinjiang Medical University, Urumqi 830000, P. R. China.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2022 Aug 25;39(4):792-797. doi: 10.7507/1001-5515.202111028.

Abstract

As the largest ecosystem of human body, intestinal microorganisms participate in the synthesis and metabolism of uric acid. Developing and utilizing intestinal bacteria to degrade uric acid might provide new ideas for the treatment of hyperuricemia. The fecal samples of people with low uric acid were inoculated into uric acid selective medium with the concentration of 1.5 mmol/L for preliminary screening, and the initially screened strains that may have degradation ability were domesticated by concentration gradient method, and the strains with high uric acid degradation rate were identified by 16S rRNA sequencing method. A strain of high-efficiency uric acid degrading bacteria was screened and domesticated from the feces of people with low uric acid. The degradation rate of uric acid could reach 50.2%. It was identified as . The isolation and domestication of high efficient uric acid degrading strains can not only provide scientific basis for the study of the mechanism of intestinal microbial degradation of uric acid, but also reserve biological strains for the treatment of hyperuricemia and gout in the future.

摘要

作为人体最大的生态系统,肠道微生物参与尿酸的合成与代谢。开发和利用肠道细菌降解尿酸可能为高尿酸血症的治疗提供新思路。将低尿酸人群的粪便样本接种到浓度为1.5 mmol/L的尿酸选择性培养基中进行初步筛选,对初步筛选出的可能具有降解能力的菌株采用浓度梯度法进行驯化,并通过16S rRNA测序法对尿酸降解率高的菌株进行鉴定。从低尿酸人群粪便中筛选驯化出一株高效尿酸降解菌,尿酸降解率可达50.2%。经鉴定为 。高效尿酸降解菌株的分离驯化不仅可为肠道微生物降解尿酸机制的研究提供科学依据,也为未来高尿酸血症和痛风的治疗储备生物菌株。

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