Li Ming, Yang Dianbin, Mei Lu, Yuan Lin, Xie Ao, Yuan Jieli
Department of Microecology, School of Basic Medical Science, Dalian Medical University, Dalian, Liaoning, China.
Department of Microecology, School of Basic Medical Science, Dalian Medical University, Dalian, Liaoning, China; Department of Gastroenterology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
PLoS One. 2014 Sep 3;9(9):e105577. doi: 10.1371/journal.pone.0105577. eCollection 2014.
Hyperuricemia is well known as the cause of gout. In recent years, it has also been recognized as a risk factor for arteriosclerosis, cerebrovascular and cardiovascular diseases, and nephropathy in diabetic patients. Foods high in purine compounds are more potent in exacerbating hyperuricemia. Therefore, the development of probiotics that efficiently degrade purine compounds is a promising potential therapy for the prevention of hyperuricemia. In this study, fifty-five lactic acid bacteria isolated from Chinese sauerkraut were evaluated for the ability to degrade inosine and guanosine, the two key intermediates in purine metabolism. After a preliminary screening based on HPLC, three candidate strains with the highest nucleoside degrading rates were selected for further characterization. The tested biological characteristics of candidate strains included acid tolerance, bile tolerance, anti-pathogenic bacteria activity, cell adhesion ability, resistance to antibiotics and the ability to produce hydrogen peroxide. Among the selected strains, DM9218 showed the best probiotic potential compared with other strains despite its poor bile resistance. Analysis of 16S rRNA sequences showed that DM9218 has the highest similarity (99%) to Lactobacillus plantarum WCFS1. The acclimated strain DM9218-A showed better resistance to 0.3% bile salt, and its survival in gastrointestinal tract of rats was proven by PCR-DGGE. Furthermore, the effects of DM9218-A in a hyperuricemia rat model were evaluated. The level of serum uric acid in hyperuricemic rat can be efficiently reduced by the intragastric administration of DM9218-A (P<0.05). The preventive treatment of DM9218-A caused a greater reduction in serum uric acid concentration in hyperuricemic rats than the later treatment (P<0.05). Our results suggest that DM9218-A may be a promising candidate as an adjunctive treatment in patients with hyperuricemia during the onset period of disease. DM9218-A also has potential as a probiotic in the prevention of hyperuricemia in the normal population.
高尿酸血症是痛风的常见病因。近年来，它还被认为是动脉粥样硬化、脑血管和心血管疾病以及糖尿病患者肾病的危险因素。富含嘌呤化合物的食物会更有效地加重高尿酸血症。因此，开发能有效降解嘌呤化合物的益生菌是预防高尿酸血症的一种有前景的潜在疗法。在本研究中，对从中国酸菜中分离出的55株乳酸菌进行了评估，以确定它们降解肌苷和鸟苷（嘌呤代谢中的两个关键中间体）的能力。在基于高效液相色谱法进行初步筛选后，选择了核苷降解率最高的三株候选菌株进行进一步表征。候选菌株的测试生物学特性包括耐酸性、耐胆汁性、抗病原菌活性、细胞黏附能力、抗生素抗性以及产生过氧化氢的能力。在所选择的菌株中，尽管DM9218的耐胆汁性较差，但与其他菌株相比，它显示出最佳的益生菌潜力。16S rRNA序列分析表明，DM9218与植物乳杆菌WCFS1的相似度最高（99%）。驯化后的菌株DM9218-A对0.3%胆盐表现出更好的抗性，并且通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）证明了其在大鼠胃肠道中的存活情况。此外，评估了DM9218-A在高尿酸血症大鼠模型中的作用。通过胃内给予DM9218-A可有效降低高尿酸血症大鼠的血清尿酸水平（P<0.05）。DM9218-A的预防性治疗比后期治疗导致高尿酸血症大鼠血清尿酸浓度降低得更多（P<0.05）。我们的结果表明，DM9218-A可能是高尿酸血症患者疾病发作期辅助治疗的一个有前景的候选菌株。DM9218-A在预防正常人群高尿酸血症方面也有作为益生菌的潜力。
