School of Pharmacy, Changzhou University, Changzhou, 213164, People's Republic of China.
Wujin Hospital Affiliated With Jiangsu University, Changzhou, 213017, Jiangsu, People's Republic of China.
Pharm Res. 2022 Oct;39(10):2475-2486. doi: 10.1007/s11095-022-03376-x. Epub 2022 Aug 25.
Gene therapy is one of the most widely studied treatments and has the potential to treat a variety of intractable diseases. The skin's limited permeability, as the body's initial protective barrier, drastically inhibits the delivery effect of gene medicine. Given the potential adverse effects and physicochemical features of the medications, improving generic drug penetration into the skin barrier and achieving an effective level of target tissues remains a challenge. Microneedles have made tremendous improvements in aided gene transfer and medication delivery as a unique method. Microneedles offer the advantage of being minimally invasive and painless, as well as the ability to distribute gene medicines straight through the stratum corneum. Microneedles have been used to penetrate skin tissue with various nucleic acids and medicines in recent years, allowing for a wide range of applications in the treatment of skin ailments. This review focuses on skin-related disorders and immunity, and it primarily discusses the progress of microneedle transdermal gene therapy in recent years. It also complements the current major vectors and related microneedle gene therapy applications.
基因治疗是研究最多的治疗方法之一，有潜力治疗多种难治性疾病。皮肤作为身体最初的保护屏障，其有限的通透性极大地抑制了基因药物的传递效果。鉴于药物的潜在不良反应和理化特性，提高普通药物穿透皮肤屏障并达到有效靶组织水平仍然是一个挑战。微针作为一种独特的方法，在辅助基因转移和药物传递方面取得了巨大的进展。微针具有微创无痛的优势，并且能够将基因药物直接递送至角质层。近年来，微针已被用于穿透皮肤组织，传递各种核酸和药物，在皮肤疾病的治疗中有广泛的应用。本综述重点介绍了与皮肤相关的疾病和免疫，并主要讨论了近年来微针经皮基因治疗的进展。它还补充了目前主要的载体和相关的微针基因治疗应用。
