尽管存在诱导的氧化应激，但预处理体育活动可能对体外受精的妊娠率产生积极影响：一项关于唾液8-羟基-2'-脱氧鸟苷的队列研究。

Pre-Treatment Physical Activity Could Positively Influence Pregnancy Rates in IVF despite the Induced Oxidative Stress: A Cohort Study on Salivary 8-Hydroxy-2'-deoxyguanosine.

作者信息

Prémusz Viktória, Lendvai-Emmert Dominika, Makai Alexandra, Amrein Krisztina, Chauhan Shalini, Bódis József, Kovács Kálmán András, Várnagy Ákos

机构信息

ELKH-PTE Human Reproduction Scientific Research Group, University of Pécs, H-7624 Pécs, Hungary.

Institute of Physiotherapy and Sport Sciences, Faculty of Health Sciences, University of Pécs, H-7621 Pécs, Hungary.

出版信息

Antioxidants (Basel). 2022 Aug 16;11(8):1586. doi: 10.3390/antiox11081586.

DOI:10.3390/antiox11081586

PMID:36009306

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9405380/

Abstract

(1) Background: This study was designed to define whether pretreatment habitual physical activity (PA)-induced oxidative stress (OS) influences outcome measures by using 8-hydroxy-2′-deoxyguanosine (8-OHdG) in saliva samples of patients undergoing in vitro fertilization (IVF). (2) Method: In this cohort study, samples were obtained from 26 patients (age: 34.6 ± 5.5 years, BMI: 25.3 ± 5.1, infertility: 51.0 ± 28.7 months) before the treatment and a follow-up of outcome measures of IVF/ICSI. The 8-OHdG was evaluated by Abcam’s ELISA (ab201734), PA patterns by GPAQ-H and ActiGraph GT3X; (3) Results: The number of matured oocytes was positively influenced by the GPAQ-H recreation MET (R2 = 0.367, F = 10.994, p = 0.004; β = 0.005, p = 0.004, B Constant = 4.604) and a positive significant relationship (R2 = 0.757, F = 17.692, p < 0.001, B Constant = 1.342) was found with GPAQ-H recreational PA MET (β = 0.004, p < 0.001), and Grade 1 embryos and higher very vigorous activity (GT3X) were accompanied (R2 = 0.958, F = 408.479, p < 0.001) by higher ß-hCG levels (β = 63.703, p ≤ 0.001). Unanticipated positive correlation between 8-OHdG and ß-hCG level (R = 0.467, p = 0.028) was noticed, and there were significant differences in 8-OHdG in biochemical pregnancies (pregnant: 54.82 ± 35.56 ng/mL, non-pregnant: 30.06 ± 10.40 ng/mL, p = 0.022) as well. (4) Conclusions: Pretreatment PA could positively influence reproductive performance in IVF/ICSI despite the induced OS. However, a more sensitive biomarker and the recommended amount of activity should be further investigated.

摘要

(1) 背景：本研究旨在通过检测体外受精（IVF）患者唾液样本中的8-羟基-2'-脱氧鸟苷（8-OHdG）来确定预处理习惯性体力活动（PA）诱导的氧化应激（OS）是否会影响结果指标。(2) 方法：在这项队列研究中，在治疗前从26名患者（年龄：34.6±5.5岁，BMI：25.3±5.1，不孕时间：51.0±28.7个月）获取样本，并对IVF/ICSI的结果指标进行随访。通过艾博抗公司的酶联免疫吸附测定法（ELISA，产品编号ab201734）评估8-OHdG，通过全球体力活动问卷简版（GPAQ-H）和ActiGraph GT3X评估PA模式；(3) 结果：成熟卵母细胞数量受到GPAQ-H休闲代谢当量（MET）的正向影响（R2 = 0.367，F = 10.994，p = 0.004；β = 0.005，p = 0.004，常数B = 4.604），并且发现与GPAQ-H休闲PA MET存在显著正相关关系（R2 = 0.757，F = 17.692，p < 0.001，常数B = 1.342）（β = 0.004，p < 0.001），1级胚胎及更高活力（GT3X）与更高的β-人绒毛膜促性腺激素（β-hCG）水平相关（R2 = 0.958，F = 408.479，p < 0.001）（β = 63.703，p≤0.001）。注意到8-OHdG与β-hCG水平之间存在意外的正相关（R = 0.467，p = 0.028），并且生化妊娠中的8-OHdG也存在显著差异（妊娠：54.82±35.56 ng/mL，未妊娠：30.06±10.40 ng/mL，p = 0.022）。(4) 结论：尽管存在诱导的OS，但预处理PA可对IVF/ICSI中的生殖性能产生积极影响。然而，应进一步研究更敏感的生物标志物和推荐的活动量。