Yang Shuting, Zhu Chuxia, Chen Jingju, Zhao Jindong, Hu Zhaoyang, Liu Shiqiang, Zhou Yong
College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China.
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China.
Biology (Basel). 2022 Jul 28;11(8):1134. doi: 10.3390/biology11081134.
Calcium ions are important second messengers, playing an important role in the signal transduction pathways. () gene family members play critical modulating roles in response to osmotic-related abiotic stress as well as other abiotic and biotic stresses, which has been reported in many plant species such as , rice, maize, and wheat. However, there has been no report about the identification and expression profile of the genes in cucumber. In this study, a total of nine genes were identified, which are unevenly distributed on the six chromosomes of cucumber. Phylogenetic analysis revealed that the OSCAs of cucumber, , rice and maize were clustered into four clades. The motif arrangement of CsOSCAs was strongly conserved, and the genes in each group had similar genetic structure. A total of 11 and 10 types of -elements related to hormone and stress, respectively, were identified in the promoter regions of genes. Gene expression analysis indicated that the genes have different expression patterns in various tissues, and some of them were regulated by three osmotic-related abiotic stresses (salt, drought and ABA) and three biotic stresses (powdery mildew, downy mildew, and root-knot nematode). As the first genome-wide identification and characterization of the gene family in cucumber, this study lays a foundation for research on the biological function and evolutionary process of this gene family, which is of great significance for exploiting stress resistant cucumber varieties.
钙离子是重要的第二信使，在信号转导途径中发挥着重要作用。（）基因家族成员在响应渗透相关的非生物胁迫以及其他非生物和生物胁迫方面发挥着关键的调节作用，这在许多植物物种如、水稻、玉米和小麦中都有报道。然而，关于黄瓜中（）基因的鉴定和表达谱尚无报道。在本研究中，共鉴定出9个（）基因，它们在黄瓜的6条染色体上分布不均。系统发育分析表明，黄瓜、水稻和玉米的OSCA基因聚为4个分支。CsOSCA基因的基序排列高度保守，每组中的（）基因具有相似的遗传结构。在（）基因的启动子区域分别鉴定出11种和10种与激素和胁迫相关的顺式作用元件。基因表达分析表明，（）基因在不同组织中具有不同的表达模式，其中一些基因受三种渗透相关的非生物胁迫（盐、干旱和脱落酸）和三种生物胁迫（白粉病、霜霉病和根结线虫）的调控。作为黄瓜中（）基因家族的首次全基因组鉴定和表征，本研究为该基因家族的生物学功能和进化过程研究奠定了基础，对培育抗逆黄瓜品种具有重要意义。
