Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca]). Members of the (L.) () gene family are reported to be involved in sensing extracellular changes to trigger hyperosmolality-induced [Ca] increases and controlling stomatal closure during immune signaling. Wheat ( L.) is a very important food crop, but there are few studies of its gene family members. In this study, 42 members were identified in the wheat genome, and phylogenetic analysis can divide them into four clades. The members of each clade have similar gene structures, conserved motifs, and domains. genes were predicted to be regulated by cis-acting elements such as STRE, MBS, DRE1, ABRE, etc. Quantitative PCR results showed that they have different expression patterns in different tissues. The expression profiles of 15 selected were examined after PEG (polyethylene glycol), NaCl, and ABA (abscisic acid) treatment. All 15 members responded to PEG treatment, while /- responded simultaneously to PEG and ABA. This study informs research into the biological function and evolution of and lays the foundation for the breeding and genetic improvement of wheat.