Harbin Normal University, Harbin, Harbin, China.
PeerJ. 2024 Mar 28;12:e17162. doi: 10.7717/peerj.17162. eCollection 2024.
The cytochrome P450 (CYP450) gene family plays a vital role in basic metabolism, hormone signaling, and enhances plant resistance to stress. Among them, the CYP82 gene family is primarily found in dicots, and they are typically activated in response to various specific environmental stresses. Nevertheless, their roles remain considerably obscure, particularly within the context of cucumber. In the present study, 12 CYP82 subfamily genes were identified in the cucumber genome. Bioinformatics analysis included gene structure, conserved motif, cis-acting promoter element, and so on. Subcellular localization predicted that all CYP82 genes were located in the endoplasmic reticulum. The results of cis element analysis showed that CYP82s may significantly affect the response to stress, hormones, and light exposure. Expression patterns of the CYP82 genes were characterized by mining available RNA-seq data followed by qRT-PCR (quantitative real-time polymerase chain reaction) analysis. Members of CYP82 genes display specific expression profiles in different tissues, and in response to PM and abiotic stresses in this study, the role of , a member of the CYP82 gene family, was investigated. The upregulation of expression in response to powdery mildew (PM) infection and treatment with methyl jasmonate (MeJA) or salicylic acid (SA) was demonstrated. Further research found that transgenic cucumber plants overexpressing display heightened resistance against PM. Wild-type (WT) leaves exhibited average lesion areas of approximately 29.7% at 7 dpi upon powdery mildew inoculation. In contrast, the two independent overexpression lines (OE#1 and OE#3) displayed significantly reduced necrotic areas, with average lesion areas of approximately 13.4% and 5.7%. Additionally, this enhanced resistance is associated with elevated expression of genes related to the SA/MeJA signaling pathway in transgenic cucumber plants. This study provides a theoretical basis for further research on the biological functions of the P450 gene in cucumber plants.
细胞色素 P450（CYP450）基因家族在基础代谢、激素信号中起着至关重要的作用，并增强了植物对胁迫的抗性。其中，CYP82 基因家族主要存在于双子叶植物中，它们通常在响应各种特定环境胁迫时被激活。然而，它们的作用仍然相当模糊，特别是在黄瓜中。本研究在黄瓜基因组中鉴定了 12 个 CYP82 亚家族基因。生物信息学分析包括基因结构、保守基序、顺式作用启动子元件等。亚细胞定位预测所有 CYP82 基因均位于内质网中。顺式元件分析的结果表明，CYP82 可能显著影响对胁迫、激素和光照的反应。通过挖掘可用的 RNA-seq 数据并进行 qRT-PCR（定量实时聚合酶链反应）分析，对 CYP82 基因的表达模式进行了表征。CYP82 基因成员在不同组织中表现出特定的表达谱，并且在本研究中对 PM 和非生物胁迫有反应，研究了 CYP82 基因家族的一个成员 的作用。研究表明，在白粉病（PM）感染和茉莉酸甲酯（MeJA）或水杨酸（SA）处理后， 的表达上调。进一步的研究发现，过表达 的转基因黄瓜植物对 PM 表现出更高的抗性。野生型（WT）叶片在白粉病接种后 7 天的平均病斑面积约为 29.7%。相比之下，两个独立的 过表达系（OE#1 和 OE#3）显示出明显较小的坏死区域，平均病斑面积约为 13.4%和 5.7%。此外，这种增强的抗性与转基因黄瓜植物中与 SA/MeJA 信号通路相关的基因的表达升高有关。本研究为进一步研究黄瓜植物中 P450 基因的生物学功能提供了理论基础。
