Ayibieke Alafate, Kobayashi Ayumi, Suzuki Masato, Sato Wakana, Mahazu Samiratu, Prah Isaac, Mizoguchi Miyuki, Moriya Kyoji, Hayashi Takaya, Suzuki Toshihiko, Iwanaga Shiroh, Ablordey Anthony, Saito Ryoichi
Department of Molecular Microbiology, Tokyo Medical and Dental University, Tokyo, Japan.
Antimicrobial Resistance Research Center, National Institute of Infectious Diseases, Tokyo, Japan.
Front Microbiol. 2020 Nov 13;11:587398. doi: 10.3389/fmicb.2020.587398. eCollection 2020.
Multidrug resistance, especially carbapenem resistance in bacteria is a global healthcare concern. However, available data on the phenotypic and genotypic characteristics of isolates from West Africa, including Ghana is scanty. Our aim was to investigate the antibiotic resistance profile and genotypic characteristics of isolates from Ghana and to characterize carbapenemase producers using whole-genome sequencing (WGS). A total of 36 isolates collected at three hospitals in Ghana between 2016 and 2017 were analyzed. MICs were determined by commercial antibiotic plates. MLST was determined using the Pasteur scheme. WGS of OXA-carbapenemase producers was performed using short- and long-read sequencing strategies. The resistance rate was highest for trimethoprim/sulfamethoxazole ( = 22; 61%). Six (16.7%) and eight (22.2%) isolates were resistant to ceftazidime and colistin, respectively. Two (5.6%) isolates were resistant and one (2.8%) isolate had intermediate sensitivity to three carbapenems. Fifteen STs were identified in 24 isolates including six new STs (ST1467 ∼ ST1472). ST78 was the predominant ( = 6) followed by ST1469 ( = 3). Four carbapenemase-producing isolates also were identified. Isogenic ST103 isolates Ab-B004d-c and Ab-D10a-a harbored within Tn on identical plasmids, pAb-B004d-c_3, and pAb-D10a-a_3. ST1472 isolate Ab-C102 and ST107 isolate Ab-C63 carried and , a rare variant, respectively, within novel genetic contexts. Our results show that isolates of diverse and unique genotypes, including OXA-carbapenemase producers, are circulating in Ghana highlighting the need for a wider surveillance of antimicrobial resistance.
多重耐药性，尤其是细菌中的碳青霉烯耐药性，是全球医疗保健领域关注的问题。然而，关于包括加纳在内的西非分离株的表型和基因型特征的现有数据很少。我们的目的是调查加纳分离株的抗生素耐药性谱和基因型特征，并使用全基因组测序（WGS）对碳青霉烯酶产生菌进行特征分析。对2016年至2017年期间在加纳三家医院收集的总共36株分离株进行了分析。通过商业抗生素平板测定最低抑菌浓度（MIC）。使用巴斯德方案确定多位点序列分型（MLST）。对产OXA-碳青霉烯酶菌株进行WGS时采用了短读长和长读长测序策略。甲氧苄啶/磺胺甲恶唑的耐药率最高（n = 22；61%）。分别有6株（16.7%）和8株（22.2%）分离株对头孢他啶和黏菌素耐药。2株（5.6%）分离株耐药，1株（2.8%）分离株对三种碳青霉烯类药物具有中介敏感性。在24株分离株中鉴定出15种序列型（ST），包括6种新的ST（ST1467～ST1472）。ST78是主要的序列型（n = 6），其次是ST1469（n = 3）。还鉴定出4株产碳青霉烯酶的分离株。同基因的ST103分离株Ab-B004d-c和Ab-D10a-a在相同质粒pAb-B004d-c_3和pAb-D10a-a_3上的转座子Tn内携带blaOXA-181。ST1472分离株Ab-C102和ST107分离株Ab-C63分别在新的基因背景下携带blaGES-5和blaNDM-1（一种罕见的blaNDM变体）。我们的结果表明，包括产OXA-碳青霉烯酶菌株在内的多种不同且独特基因型的分离株在加纳传播，这突出表明需要更广泛地监测抗菌药物耐药性。
