Zhao Lina, Xia Xinxin, Yuan Ting, Zhu Junying, Shen Zhen, Li Min
Department of Laboratory Medicine, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China.
Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR 999077, China.
Antibiotics (Basel). 2022 Aug 13;11(8):1100. doi: 10.3390/antibiotics11081100.
This study analyzed genomic data of 4643 strains of carbapenemase-carrying (KPN) in China by using the Kleborate software package. The data showed rich diversity in carbapenemase-carrying KPN genomes, which contain not only 152 sequence types but also 90 capsular serotypes. In 2013, the transfer of carbapenemase to hypervirulent (HvKP) of KL1 and KL2 occurred, and since 2014, the propagation of carbapenemase into mammals, poultry, and insects has been detected. The ST11 capsular serotype had a reversal of the prevalence of KL47 and KL64 in 2016, with KL64 replacing KL47 as the dominant serotype. Colibactin is a very suitable indicator to differentiate KL1-type HvKP and classic Klebsiella pneumoniae. The most prevalent yersiniabactin of KL1 is ICEKp10, and that of ST11 carbapenem-resistant KPN(ST11-CRKP) is ICEKp3. The virulence genes of KL1 carbapenem-resistant hypervirulent KPN (KL1-CRHvKP), as well as ST65- and ST86-type KL2-CRHvKP, were not lost after carbapenemase was obtained.
本研究使用Kleborate软件包分析了中国4643株携带碳青霉烯酶的肺炎克雷伯菌（KPN）的基因组数据。数据显示，携带碳青霉烯酶的KPN基因组具有丰富的多样性，不仅包含152种序列类型，还包含90种荚膜血清型。2013年，碳青霉烯酶转移至KL1和KL2的高毒力（HvKP）菌株中，自2014年以来，已检测到碳青霉烯酶在哺乳动物、家禽和昆虫中的传播。ST11荚膜血清型在2016年出现了KL47和KL64流行率的逆转，KL64取代KL47成为优势血清型。大肠杆菌素是区分KL1型HvKP和经典肺炎克雷伯菌的非常合适的指标。KL1最普遍的yersiniabactin是ICEKp10，而ST11耐碳青霉烯KPN（ST11-CRKP）的是ICEKp3。KL1耐碳青霉烯高毒力KPN（KL1-CRHvKP）以及ST65和ST86型KL2-CRHvKP的毒力基因在获得碳青霉烯酶后并未丢失。
