Departament de Genètica, Microbiologia i Estadística, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain.
Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Baldiri Reixac, 10, 08028 Barcelona, Spain.
Cells. 2022 Aug 16;11(16):2542. doi: 10.3390/cells11162542.
The loss-of-function conditions for an () in larvae reared at 29 °C results in malignant brain tumours and hyperplastic imaginal discs. Unlike the former that have been extensively characterised, little is known about the latter. Here we report the results of a study of the hyperplastic mutant wing imaginal discs. We identify the wing disc tumour transcriptome and find it to include genes involved in reactive oxygen species (ROS) metabolism. Furthermore, we show the presence of oxidative stress in hyperplastic discs, even in apoptosis-blocked conditions, but not in brain tumours. We also find that chemically blocking oxidative stress in wing discs reduces the incidence of wing disc overgrowths. Our results reveal the involvement of oxidative stress in wing discs hyperplastic growth.
在 29°C 下饲养的幼虫中，()的功能丧失会导致恶性脑瘤和过度增生的想象盘。与前者被广泛描述不同，后者知之甚少。在这里，我们报告了对过度增生的 突变体翅膀想象盘的研究结果。我们确定了 翅膀盘肿瘤转录组，并发现其中包括参与活性氧 (ROS) 代谢的基因。此外，我们还发现即使在阻止凋亡的情况下，过度增生的 翅膀盘中也存在氧化应激，但在 脑瘤中则没有。我们还发现，在 翅膀盘中用化学方法阻断氧化应激可降低翅膀盘过度生长的发生率。我们的结果表明氧化应激参与了 翅膀盘的过度增生。
