Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Center for Genomics and Bio-Computing, School of Life Sciences, North China University of Science and Technology, Tangshan 063210, China.
Int J Mol Sci. 2022 Aug 12;23(16):8986. doi: 10.3390/ijms23168986.
Celery ( L.), a plant from Apiaceae, is one of the most important vegetables and is grown worldwide. Carotenoids can capture light energy and transfer it to chlorophyll, which plays a central role in photosynthesis. Here, by performing transcriptomics and genomics analysis, we identified and conducted a comprehensive analysis of chlorophyll and carotenoid-related genes in celery and six representative species. Significantly, different contents and gene expression patterns were found among three celery varieties. In total, 237 and 290 chlorophyll and carotenoid-related genes were identified in seven species. No notable gene expansion of chlorophyll biosynthesis was detected in examined species. However, the gene encoding ζ-carotene desaturase (ZDS) enzyme in carotenoid was expanded in celery. Comparative genomics and RNA-seq analyses revealed 16 and 5 key genes, respectively, regulating chlorophyll and carotenoid. An intriguing finding is that chlorophyll and carotenoid-related genes were coordinately regulated by transcriptional factors, which could be distinctively classified into positive- and negative-regulation groups. Six ()-like transcription factors co-regulated chlorophyll and carotenoid-related genes were identified in celery. In conclusion, this study provides new insights into the regulation of chlorophyll and carotenoid by transcription factors.
芹菜（L.）是伞形科的一种植物，是最重要的蔬菜之一，在世界各地广泛种植。类胡萝卜素可以捕获光能并将其转移到叶绿素中，叶绿素在光合作用中起着核心作用。在这里，通过进行转录组学和基因组学分析，我们鉴定并对芹菜和六种代表性物种中的叶绿素和类胡萝卜素相关基因进行了全面分析。值得注意的是，在三种芹菜品种中发现了不同的含量和基因表达模式。在七种物种中共鉴定出 237 个和 290 个与叶绿素和类胡萝卜素相关的基因。在检查的物种中，没有发现叶绿素生物合成的明显基因扩张。然而，在类胡萝卜素中编码 ζ-胡萝卜素去饱和酶（ZDS）酶的基因在芹菜中得到了扩展。比较基因组学和 RNA-seq 分析分别揭示了分别调节叶绿素和类胡萝卜素的 16 个和 5 个关键基因。一个有趣的发现是，转录因子协调调节叶绿素和类胡萝卜素相关基因，这些基因可以分为正调控和负调控两个不同的组。在芹菜中鉴定出六个（）样转录因子共同调控叶绿素和类胡萝卜素相关基因。总之，本研究为转录因子对叶绿素和类胡萝卜素的调节提供了新的见解。
